DNA ke eng?

Sebopeho le Sebopeho sa DNA

DNA, eo hape e tsejoang, e le deoxyribonucleic acid ke molek'hule, e leng sehlopha sa liathomo tse khomaretsoeng hammoho.Tabeng ea DNA, liathomo tsena lia kopanngoa ho etsa sebōpeho sa lere e telele e bilikang.Re ka bona setšoantšo mona ka ho hlaka ho lemoha sebōpeho sa DNA.

Haeba u kile ua ithuta baeloji, mohlomong u ile ua utloa hore DNA e sebetsa e le polane kapa risepe ea lintho tse phelang.Ke joang lefatšeng molek’hule feela e ka sebetsang e le polane ea ntho e rarahaneng le e babatsehang joaloka sefate, ntja le batho?Hoa hlolla ruri.

DNA ke e 'ngoe ea litataiso tsa bohlokoahali.E rarahane ho feta buka efe kapa efe eo u kileng oa e sebelisa.Tataiso eohle ea litaelo e ngotsoe ka khoutu.Haeba u sheba sebopeho sa lik'hemik'hale tsa DNA haufi-ufi, se tla bontša likarolo tse 'nè tsa mohaho.Re bitsa metheo ena ea naetrojene: Adenine (A), Thymine (T), Guanine (G), le Cytosine (C).DNA e boetse e kenyelletsa lihlopha tsa tsoekere le phosphate (tse entsoeng ka phosphorus le oksijene).Tsena li etsa mokokotlo oa phosphate-deoxyribose.

Haeba u nahana ka sebopeho sa DNA e le lere, lithapo tsa lere li entsoe ka metheo ea naetrojene.Lithako tsena li kopana ho etsa mohato o mong le o mong oa lere.Li boetse li kopantsoe ka mokhoa o ikhethileng.(A) kamehla li kopane le (T) le (G) kamehla li kopane le (C).Sena se bohlokoa haholo ha e le nako ea ho kopitsa DNA eohle kapa karolo ea eona.

Kahoo, ho araba potso, DNA ke eng?DNA ke polane ea limolek'hule bakeng sa ntho e phelang.DNA e hlahisa RNA, ’me RNA e hlahisa protheine, ’me liprotheine li tsoela pele ho etsa bophelo.Ts'ebetso ena kaofela e rarahane, e rarahane ebile e na le boselamose 'me e thehiloe ka ho feletseng k'hemistri e ka ithutoang le ho utloisisoa.

Mokhoa oa ho arola DNA Fragment?

JOALOKAHA re boletse hore DNA e ka ithutoa le ho utloisisoa, empa re ka e etsa joang?Bo-rasaense baa ithuta le ho etsa lipatlisiso le ho li hlahloba.Batho ba sebelisa gel electrophoresis ho arola DNA bakeng sa lipatlisiso tse ling.Gel electrophoresis ke mokhoa o sebelisoang ho arola likaroloana tsa DNA (kapa li-macromolecules tse ling, tse kang RNA le liprotheine) ho latela boholo ba tsona le tefiso.Electrophoresis e kenyelletsa ho tsamaisa motlakase ka gel e nang le limolek'hule tsa thahasello.Ho itšetlehile ka boholo ba tsona le tefiso ea tsona, limolek'hule li tla tsamaea ho pholletsa le gel ka litsela tse sa tšoaneng kapa ka lebelo le fapaneng, ho li lumella hore li arohane ho tse ling.Ka ho sebelisa electrophoresis, re khona ho bona hore na ke likarolo tse kae tse fapaneng tsa DNA tse teng sampuling le hore na li boholo hakae ha li bapisoa le tse ling.

Haeba u batla ho etsa gel electrophoresis, pele u hloka lisebelisoa tse amanang le liteko, sele ea electrophoresis (tanka / kamore) le matla a eona.Setšoantšo se latelang se bontša sele e otlolohileng ea electrophoresis (tanka / kamore) ea mohlalaDYCP-31DNle mohlala oa phepelo ea motlakaseDYY-6Dho tsoa Beijing Liuyi Biotechnology Co., Ltd bakeng sa DNA gel electrophoresis.

Gel electrophoresis e kenyelletsa gel, e leng mofuta oa thepa e kang Jello.Li-gel bakeng sa karohano ea DNA hangata li sebelisoa agarose, e tlang e le li-flakes tse omeletseng, tse phofshoana.Ha agarose e futhumatsoa ka har'a buffer (metsi a nang le matsoai a mang ka har'a eona) 'me e lumelloa ho pholile, e tla etsa gel e tiileng, e fokolang hanyenyane.Boemong ba limolek'hule, gel ke matrix a limolek'hule tsa agarose tse kopantsoeng hammoho ke li-hydrogen bond ebe li etsa masoba a manyenyane.

Setšoantšo se tsoang ho Khan Academy

Kamora ho lokisa gel, kenya gel ka har'a tanka ea sele ea electrophoresis, 'me u tšollele tharollo ea buffer ka tanka ea buffer ho fihlela gel e qoelisoa.Ebe lisampole tsa DNA li kenngoa ka lilibeng (li-indentations) ka lehlakoreng le leng la gel, ebe ho sebelisoa motlakase ho li hula ka gel.Likaroloana tsa DNA li qosoa hampe, kahoo li fallela ho electrode e ntle.Hobane likaroloana tsohle tsa DNA li na le tefiso e lekanang ka boima ka 'ngoe, likhechana tse nyane li tsamaea ka gel kapele ho feta tse kholo.Ka mor'a ho tsamaisa gel electrophoresis, likaroloana tsa DNA li arohane;'me bafuputsi ba ka hlahloba gel le ho bona hore na ho na le lihlopha tse kae tse fumanoang ho eona.Ha gel e silafalitsoe ka dae e tlamang DNA 'me e behoa tlas'a leseli la UV, likhechana tsa DNA li tla khanya, li re fa monyetla oa ho bona DNA e teng libakeng tse fapaneng ho latela bolelele ba gel.

Ntle le lisele tsa electrophoresis (litanka / likamore) le lisebelisoa tsa motlakase, Beijing Liuyi Biotechnology Co., Ltd e boetse e fana ka transilluminator ea UV, e ka bonang le ho nka linepe bakeng sa protheine le gel ea electrophoresis ea DNA.MohlalaWD-9403Bke transilluminator e nkehang ea UV bakeng sa ho shebella gel ea DNA electrophoresis.MohlalaWD-9403Fka hlokomela, nka linepe bakeng sa protheine le gel ea DNA.