Mokhoa oa ho etsa DNA Electrophoresis ka Agarose Gel?

Mona re tla hlalosa mokhoa oa ho etsa agarose gel electrophoresis ke mofuputsi oa rona lab ea Liuyi Biotech.

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Pele ho tlhahlobo, re hloka ho lekola lisebelisoa, li-reagents le lisebelisoa tse ling tsa liteko le lisebelisoa tseo re li hlokang.

Tokisetso ea lisebelisoa tsa liteko le lisebelisoa

Lisebelisoa tsa agarose gel electrophoresis

Horizontal electrophoresis cell(tanka/kamore), phepelo ea motlakase ea electrophoresis e bohareng le e tlase, setšoantšo sa gel le sistimi ea tlhahlobo.

Beijing liuyi Biotechnology Co., Ltd (Liuyi Biotech) e fana ka mefuta e fapaneng ea sele e otlolohileng ea electrophoresis (tanka/kamore) le phepelo ea motlakase hammoho le sistimi ea litokomane tsa gel.Lihlahisoa tsa letoto la DYCP-31 ke tsa electrophoresis, 'me lihlahisoa tsa letoto la DYY ke phepelo ea motlakase ea electrophoresis.Lihlahisoa tsa letoto la WD-9413 ke sistimi ea litokomane tsa gel.Ho tloha ka boholo ba gel e nyane 60 × 60mm ho isa ho gel e kholo 250 × 250mm, re ka fihlela litlhoko tsa hau tse fapaneng tsa boholo ba gel.MohlalaDYCP-32Ce ka etsa hore gel e fihle boholo ba 250×250mm.TheDYY-6Cke matla a rona.E ts'ehetsa tlhahiso ea 400V, 400mA, 240W, e leng sehlahisoa sa rona se tloaelehileng se sebelisoang ke bareki ba rona. WD-9413BGel Image & Analysis System e sebelisetsoa ho hlahloba le ho etsa lipatlisiso bakeng sa gel, lifilimi le li-blots ka mor'a teko ea electrophoresis.Ke sesebelisoa sa mantlha se nang le mohloli oa leseli la ultraviolet bakeng sa ho bona le ho nka lifoto tsa li-gel tse silafalitsoeng ka lidae tse fluorescent joalo ka ethidium bromide, le mohloli o mosoeu oa leseli bakeng sa ho bona le ho nka lifoto lijelle tse silafalitsoeng ka lidae tse kang coomassie boputsoa bo khanyang.

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Li-reagents tsa agarose gel electrophoresis

1.The medium: agarose gel

2.The buffer: TAE (Tris-acetate, EDTA, glacial acetic acid) le TBE (Tris-borate, EDTA, boric acid).

3.The loading buffer: 6×DNA Loading Buffer (Special for DNA sample: EDAT, glycerine,Xylene cyanol le bromophenol blue)

The Dye

Dae ea fluorescent joalo ka EB, Gelred, goldview, GenGreen, GenView, SYBRGreen

TheLintho tse ka sebelisoang

Malebela a ho sterilization pipette (10μL), malebela a pipette (200μL), malebela a pipette (1000μL), 200μL\500μL\1.5ml centrifuge tube.

Letšoao la DNA

E lokiselitsoe ho latela boima ba molek'hule e lokelang ho lekoa.

Gel e matha le ho shebella

Pele, re hloka ho lokisa gel ea agarose.Motsoako oa agarose ka gel o tla itšetleha ka boholo ba likhechana tsa DNA tse lokelang ho aroloa, 'me boholo ba li-gel li tloha pakeng tsa 0.5% -2%.Ho nka tsa ronaDYCP-31DNmohlala, O ka lahlela gel e nyane, gel e pharaletseng, gel e telele le gel e sekoere ho latela litlhoko tsa teko.Khetha tray ea gel eo ue hlokang, 'me u kenye kama, ebe u tšela gel e futhumetseng ea agarose ka har'a sesebelisoa sa ho lahlela gel.

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Joale, ka mor'a hore gel e tiee, tlosa kama ka hloko le ka bonolo, 'me u behe terei ea gel ka tankeng ea buffer.Tšela tharollo ea "buffer" ka tanka ea "buffer" 'me u etse hore gel eohle e qoelisoe ka har'a buffer.Laela lisampole ka har'a liliba ka pipette e tloaelehileng.Connect theDYCP-31DNka motlakase oa electrophoresis ka mokhoa o nepahetseng, 'me u behe mekhoa ea ho tsamaisa gel.

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Bona ka mahlo likotoana tsa DNA ea hau ho transilluminator ea UV.

AKamora ho tsamaisa gel, o ka sebelisa setšoantšo sa rona sa gel le mofuta oa tlhahlobo ea sistimiWD-9413Bho shebella, ho sekaseka le ho nka litšoantšo tsa gel.Liuyi Biotech e boetse e fana ka UV transilluminator (UV Analyzer) ho shebella gel.Re na le black-box mofuta oa UV transilluminator (UV Analyzer) mohlalaWD-9403A, 9403C, WD-9403F, mohlala o nkehang habobebe oa UV transilluminator (UV Analyzer).WD-9403Ble ho tšoara transilluminator ea UV (UV Analyzer)WD-9403Ehobane u khetha.

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Setšoantšo sa gel post electrophoresis

Letšoao la Liuyi le na le nalane ea lilemo tse fetang 50 Chaena mme k'hamphani e ka fana ka lihlahisoa tse tsitsitseng le tsa boleng bo holimo lefatšeng ka bophara.Ho theosa le lilemo tsa nts'etsopele, e loketse khetho ea hau!

Ho fumana lintlha tse ling ka rona, ka kopo ikopanye le rona ka lengolo-tsoibila[imeile e sirelelitsoeng], [imeile e sirelelitsoeng].


Nako ea poso: Apr-29-2022