Mona re tla hlalosa mokhoa oa ho etsa agarose gel electrophoresis ke mofuputsi oa rona lab ea Liuyi Biotech.
Pele ho tlhahlobo, re hloka ho lekola lisebelisoa, li-reagents le lisebelisoa tse ling tsa liteko le lisebelisoa tseo re li hlokang.
Tokisetso ea lisebelisoa tsa liteko le lisebelisoa
Lisebelisoa tsa agarose gel electrophoresis
Horizontal electrophoresis cell(tanka/kamore), phepelo ea motlakase ea electrophoresis e bohareng le e tlase, setšoantšo sa gel le sistimi ea tlhahlobo.
Beijing liuyi Biotechnology Co., Ltd (Liuyi Biotech) e fana ka mefuta e fapaneng ea sele ea electrophoresis (tanka/kamore) le phepelo ea motlakase hammoho le sistimi ea litokomane tsa gel. Lihlahisoa tsa letoto la DYCP-31 ke tsa electrophoresis, 'me lihlahisoa tsa letoto la DYY ke phepelo ea motlakase ea electrophoresis. Lihlahisoa tsa letoto la WD-9413 ke sistimi ea litokomane tsa gel. Ho tloha ka boholo bo bonyenyane ba gel 60 × 60mm ho ea ho gel e kholo 250 × 250mm, re ka finyella litlhoko tsa hau tse fapaneng tsa boholo ba gel. MohlalaDYCP-32Ce ka etsa hore gel e fihle boholo ba 250×250mm. TheDYY-6Cke matla a rona. E ts'ehetsa tlhahiso ea 400V, 400mA, 240W, e leng sehlahisoa sa rona se tloaelehileng se sebelisoang ke bareki ba rona. WD-9413BGel Image & Analysis System e sebelisetsoa ho hlahloba le ho etsa lipatlisiso bakeng sa gel, lifilimi le li-blots ka mor'a teko ea electrophoresis. Ke sesebelisoa sa mantlha se nang le mohloli oa leseli la ultraviolet bakeng sa ho bona le ho nka lifoto tsa li-gel tse silafalitsoeng ka lidae tsa fluorescent joalo ka ethidium bromide, le mohloli o mosoeu oa leseli bakeng sa ho bona le ho nka lifoto tsa li-gel tse silafalitsoeng ka lidae tse kang coomassie boputsoa bo botala.
Li-reagents tsa agarose gel electrophoresis
1.The medium: agarose gel
2.The buffer: TAE (Tris-acetate, EDTA, glacial acetic acid) le TBE (Tris-borate, EDTA, boric acid).
3.The loading buffer: 6×DNA Loading Buffer (Special for DNA sample: EDAT, glycerine,Xylene cyanol le bromophenol blue)
The Dye
Dae ea fluorescent joalo ka EB, Gelred, goldview, GenGreen, GenView, SYBRGreen
TheLintho tse ka sebelisoang
Malebela a ho sterilization pipette (10μL), malebela a pipette (200μL), malebela a pipette (1000μL), 200μL\500μL\1.5ml centrifuge tube.
Letšoao la DNA
E lokiselitsoe ho latela boima ba molek'hule e lokelang ho lekoa.
Gel e matha le ho shebella
Pele, re hloka ho lokisa gel ea agarose. Motsoako oa agarose ka gel o tla itšetleha ka boholo ba likhechana tsa DNA tse lokelang ho aroloa, 'me boholo ba li-gel li tloha pakeng tsa 0.5% -2%. Ho nka tsa ronaDYCP-31DNmohlala, O ka lahlela gel e nyane, gel e pharaletseng, gel e telele le gel e sekoere ho latela litlhoko tsa teko. Khetha tray ea gel eo ue hlokang, 'me u kenye kama, ebe u tšela gel e futhumetseng ea agarose ka har'a sesebelisoa sa ho lahla gel.
Joale, ka mor'a hore gel e tiee, tlosa kama ka hloko le ka bonolo, 'me u behe terei ea gel ka tanka ea buffer. Tšela tharollo ea "buffer" ka tanka ea "buffer" 'me u qoelise gel eohle ka har'a buffer. Laela lisampole ka har'a liliba ka pipette e tloaelehileng. Connect theDYCP-31DNka motlakase oa electrophoresis ka nepo, 'me u behe mekhoa ea ho tsamaisa gel.
Bona ka mahlo likotoana tsa DNA ea hau ho transilluminator ea UV.
AKamora ho tsamaisa gel, o ka sebelisa setšoantšo sa rona sa gel le mofuta oa tlhahlobo ea sistimiWD-9413Bho shebella, ho sekaseka le ho nka litšoantšo tsa gel. Liuyi Biotech e boetse e fana ka UV transilluminator (UV Analyzer) ho shebella gel. Re na le black-box mofuta oa UV transilluminator (UV Analyzer) mohlalaWD-9403A, 9403C, WD-9403F, mohlala o nkehang habobebe oa UV transilluminator (UV Analyzer).WD-9403Ble ho tšoara transilluminator ea UV (UV Analyzer)WD-9403Ehobane u khetha.
Setšoantšo sa gel post electrophoresis
Letšoao la Liuyi le na le nalane ea lilemo tse fetang 50 Chaena mme k'hamphani e ka fana ka lihlahisoa tse tsitsitseng le tsa boleng bo holimo lefatšeng ka bophara. Ho theosa le lilemo tsa nts'etsopele, e loketse khetho ea hau!
Ho fumana lintlha tse ling ka rona, ka kopo ikopanye le rona ka lengolo-tsoibila[imeile e sirelelitsoeng], [imeile e sirelelitsoeng].
Nako ea poso: Apr-29-2022