Agarosagel electrophoresis
Agarose gel electrophoresis ke mokhoa oa gel electrophoresis o sebelisoang ho biochemistry, biology ea molek'hule, liphatsa tsa lefutso le k'hemistri ea bongaka ho arola palo e tsoakaneng ea li-macromolecules tse kang DNA kapaRNA. Sena se finyelloa ka ho tsamaisa limolek'hule tsa nucleic acid tse nang le qoso e mpe ka matrix a agarose leelectrophoresis.
Thepa ea gel ea agarose
Agarose gel ke matrix a mahlakore a mararo a entsoeng ka limolek'hule tsa helical agarose ka mekotla e meholo e kopantsoeng ka likarolo tse tharo tse nang le likanale le li-pores tseo li-biomolecules li ka fetang ho tsona. ka ho futhumatsa ho kgutlela boemong bo metsi.Mocheso oa ho qhibiliha o fapane le mocheso oa gelling, ho itšetlehile ka mehloli, gel ea agarose e na le mocheso oa gelling oa 35.-42 °C le mocheso o qhibilihang oa 85-95 °C.Li-agarose tse qhibilihang ka tlaase le tse tlaase tse entsoeng ka liphetoho tsa lik'hemik'hale li fumaneha hape.
Melemo le melemo ea agarose gel electrophoresis
Molemo o ka sehloohong oa mokhoa oa agarose gel electrophoresis ke hore o ka sebetsoa habonolo mme molek'hule ea DNA e sebelisoang e le sampole le eona e ka khutlisoa ntle le kotsi ho eona qetellong ea ts'ebetso.Hobane gel ea agarose ha e sebetse'Ha e na sehlopha sa sulfate le carboxyl, e ke ke ea baka electroosmosis ea agar.Ka sebopeho se ts'oanang le boholo ba pore e kholo, gel ea agarose e ntle bakeng saho arola limolek'hule tse kholo tsa DNA, joalo ka enzyme e rarahaneng, nucleic acid le kokoana-hloko.Gel ea Agarose ha e sebetse'Ho monya leseli la UV, kahoo e ka silafatsoa ka kotloloho kapa ka mor'a hore e be filimi, e ka fetoloa hape ka mocheso.
Ho boetse ho na le mathata a mang a mokhoa oa agarose gel electrophoresis.Gel ea Agarose e robeha habonolo, kahoo boima bo ke ke ba fokotseha haholo, bo tla bitsa agarose e eketsehileng.Gel ea agarose ho thata ho e boloka hobane ho bonolo ho silafatsoa ke baktheria, 'me e lokela ho lokisoa pele e e sebelisa.Ha ho bapisoa le mokhoa oa PAGE electrophoresis, katleho ea sieve ea molek'hule e fokotsehile, le sebaka sa electrophoresis se fokotseha.
E tshekaletsengelectrophoresistsamaiso ea gel ea agarose
Ntle le agarose le buffer, teko ea agarose gel electrophoresis e hloka sistimi e otlolohileng ea electrophoresis le maemo a tlase.motlakase oa motlakase oa electrophoresis.Beijing Liuyi Biotechnology e ka fana ka sistimi e otlolohileng ea electrophoresis le motlakase oa electrophoresis, joalo ka mohlalaDYCP-31BN, DYCP-31CN bakeng satsamaiso e otlolohileng ea electrophoresis leDYY-6C bakeng sa phepelo ea motlakase.
Ho ipapisitse le ka boholo bo fapaneng ba li-gele hlokehang ke Teko ea agrose gel electrophoresis, boholo bo fapaneng ba sistimi ea electrophoresis e otlolohileng e ka fanoa ke Beijing Liuyi Biotechnology.Letoto la lihlooho tsa DYCP-31,sjoalo kaDYCP-31BN, DYCP-31CN, DYCP-31DN, DYCP-31E, DYCP-32B, leDYCP-32Cba na le boholo bo fapaneng ba tray ea gel, ho tloha ho lahla gel e nyane ho isa ho gel e kholo, ba ka kopana le liteko tsa hau.ditlhoko.
Letšoao la Liuyi le na le nalane ea lilemo tse fetang 50 Chaena mme k'hamphani e ka fana ka lihlahisoa tse tsitsitseng le tsa boleng bo holimo lefats'eng lohle.Nts'etsopele ea lilemo, e tšoaneloa ke khetho ea hau!
Ho fumana lintlha tse ling ka rona, ka kopo ikopanye le rona ka lengolo-tsoibila[imeile e sirelelitsoeng], thekiso01@ly.com.cn.
Nako ea poso: Apr-16-2022