DNA gel electrophoresis ke mokhoa o tloaelehileng oa baeloji ea limolek'hule o sebelisetsoang ho arola le ho hlahloba likhechana tsa DNA ho latela boholo ba tsona.Ts'ebetso ena e kenyelletsa ho kenya likhechana tsa boholo bo fapaneng tsa DNA gel e entsoeng ka agarose, k'habohaedreite e fumanoang boleleng bo bofubelu.
Ho lokisa le ho lahlela gel ea agarose
- Qhala agarose ka tekanyo e loketseng ea electrophoresis buffer.Boikoetliso ba gel bo khethoa ke karo-karolelano ea boima ho ea ho bophahamo, joalo ka 1g ea agarose ho 100.ml ea buffer bakeng sa gel ea 1%.
- Chesa motsoako ka microwave, o potoloha setshelo ho netefatsa hore agarose e felile.
- Eketsa ethidium bromide ho tharollo ea gel sebakeng sa ho qetela sa 0.5mg/ ml.Ethidium bromide e hokahana lipakeng tsa lipara tse haufi tsa DNA ebe e ntša orange fluorescence tlas'a leseli la UV.Hlokomela hore ethidium bromide ke carcinogen, kahoo ho e tšoara ho hloka mehato e nepahetseng ea tšireletso joaloka ho roala liatlana.
- Folisa tharollo ea gel ka bateng ea metsi ho thibela terei ea gel hore e se ke ea soahlamana ka lebaka la mocheso o phahameng.
- Beha kama ka har'a tharollo ea gel ho etsa mohlala oa liliba.Khetha li-combs tse loketseng palo ea sampole ea DNA eo u tla e kenya.Tšela tharollo ea gel ea agarose ka har'aterei ea gel'me u e lumelle ho tiisa mocheso oa kamore.
- Hang ha gel e se e tiile, tlosa khekhe.Haeba u tla be u sa sebelise gel hang-hang, e phuthele ka sekoahelo sa polasetiki 'me ue boloke ho 4℃ ho fihlela ho hlokahala.
Ho lokisa le ho tsamaisa gel
Pele o qala electrophoresis, kopanya sampole ea DNA le buffer ea ho kenya.Hangata, "buffer" ea ho kenya e tsepamisitsoe ka makhetlo a tšeletseng ho feta 'me e thusa ho teba ha sampuli ho ea tlaase ho liliba le ho lekola motsamao nakong ea electrophoresis.
Beha phepelo ea motlakase ho voltage e boletsoeng.
Eketsa buffer e lekaneng ea electrophoresis ho tanka ea gel ho koahela bokaholimo ba gel.Netefatsalikhokahano tse nepahetseng tsa li-electrode.
Laola sampole ea DNA le matšoao a boima ba limolek'hule lilibeng tsa gel.
Bulela phepelo ea motlakase ho qala electrophoresis.
Ho shebella likaroloana tse arohaneng tsa DNA
Ka mor'a electrophoresis, tima motlakase 'me u tlose gel.
Sebelisa mohloli oa lebone la UV ho khantša gel;Likhechana tsa DNA li tla hlaha e le lihlopha tsa orange fluorescent.
Ngola setšoantšo sa gel ho hlahloba likhechana tsa DNA tse arohaneng.
Ka mor'a teko, etsa bonnete ba ho lahla gel le electrophoresis buffer hantle, ho latela tataiso ea laboratori.Kamehla u roale liatlana ha u tšoara li-gel le li-buffer tse nang le ethidium bromide ho itšireletsa khahlanong le ho pepesehela ntho ena e kotsi.
DNA gel electrophoresis e sebelisoa haholo ho baeloji ea limolek'hule le lipatlisiso tsa lefutso bakeng sa ho hakanya boholo ba limolek'hule tsa DNA, ho arola likhechana tsa DNA, ho bona liphetoho tsa lefutso, le khatiso ea menoana ea DNA, har'a lits'ebetso tse ling.Ke mokhoa o bonolo le o sebetsang oa liteko o thusang ho utloisisa sebopeho le litšobotsi tsa disampole tsa DNA.
Beijing Liuyi Biotechnology Co.Ltd, moetsi ea hloahloa ea shebaneng le lihlahisoa tsa electrophoresis ho feta lilemo tse 50, o fana ka mefuta e mengata ea litanka tsa electrophoresis (liphaposi / lisele) bakeng sa gel electrophoresis, joalo ka litanka tse otlolohileng tsa electrophoresis (liphaposi / lisele) bakeng saDNAgel electrophoresis, le litanka tse otlolohileng tsa electrophoresis (liphaposi / lisele) bakeng sa protheine gel electrophoresis.Ho sa le joalo, e boetse e fana ka mefuta e fapaneng ea phepelo ea motlakase ea electrophoresis, analyzer ea lebokose le lefifi la khabinete le transilluminator ea UV.U ka khetha litanka tsa Beijing Liuyi tsa electrophoresis (liphaposi/lisele) ho lekanya boima ba limolek'hule tsa RNA le ho arola RNA ea boholo bo fapaneng bakeng sa lab ea hau.
Mona re tlakhothaletsamofuta o mong oa e tshekaletseng electrophoresis tanka (kamoreng / sele) mohlalaDYCP-31DN ho etsa le ho tsamaisa gel.
Kamora ho tsamaisa gel, o ka sebelisa setšoantšo sa rona sa gel le mofuta oa tlhahlobo ea sistimiWD-9413Bho shebella, ho sekaseka le ho nka litšoantšo tsa gel.Liuyi Biotech e boetse e fana ka UV transilluminator (UV Analyzer) ho shebella gel.Re na le mofuta oa lebokose le letšo la mofuta oa UV transilluminator (UV Analyzer).WD-9403A,9403C,WD-9403F, mohlala o nkehang habobebe oa UV transilluminator (UV Analyzer).WD-9403Ble ho tšoara transilluminator ea UV (UV Analyzer)WD-9403Ehobane u khetha.
Letšoao la Beijing Liuyi le na le nalane ea lilemo tse fetang 50 Chaena mme k'hamphani e ka fana ka lihlahisoa tse tsitsitseng le tsa boleng bo holimo lefatšeng ka bophara.Ho theosa le lilemo tsa nts'etsopele, e loketse khetho ea hau!
Hona joale re batla balekane, bobeli ba OEM electrophoresis tanka le baabi ba amoheloa.
Haeba u na le moralo oa ho reka lihlahisoa tsa rona, ka kopo u se ke oa qeaqea ho ikopanya le rona.U ka re romella molaetsa ka lengolo-tsoibila[imeile e sirelelitsoeng]kapa[imeile e sirelelitsoeng], kapa ka kopo re letsetse ho +86 15810650221 kapa eketsa Whatsapp +86 15810650221, kapa Wechat: 15810650221.
Nako ea poso: Aug-08-2023