Cellulose Acetate Membrane Electrophoresis ke eng?
Cellulose Acetate Membrane Electrophoresis ke mofuta o mong oa mekhoa ea electrophoresis e sebelisang lera la cellulose acetate e le mecha ea litaba e tšehetsang liteko.
Cellulose Acetate ke mofuta oa acetate ea selulose e leng acetylated ho tsoa ho cellulose's hydroxyl.Ha e qhibiliha ka har'a tharollo ea manyolo joalo ka acetone, e ka koaheloa ho filimi e ts'oanang le e ntle e nang le li-micro-pores.Botenya ba filimi bo pota-potile 0.1-0.15mm e loketseng liteko.
Melemo ea cellulose acetate membrane
Ha ho bapisoa le pampiri ea fliter, e na le melemo e latelang:
1.Phello e ntle ea karohano.Bakeng sa lisampole tsa protheine, lera la cellulose acetate le monya tse 'maloa tsa tsona, ka mor'a dae, li ka senyeha ka ho felletseng ho tsoa ho' mala o ka morao.Lihlopha tsa dae li hlakile haholo, kahoo li khothalletsa ho nepahala bakeng sa qeto ea palo.
2.Fast le ho boloka nako.Lera la cellulose acetate ha le na hydyophilic ho feta pampiri ea fliter, kahoo ts'ebetso ea electro-osmosis e tlase ho pampiri ea fliter, 'me motlakase o tsamaisoa haholo ke lisampole tse ka khutsufatsang nako ea karohano le ho fokotsa nako ea electrophoresis.Ka tloaelo, nako ea cellulose acetate membrane electrophoresis e ka bang 45-60minutes.Ts'ebetso eohle ea electrophoresis ho kenyelletsa ho daea le ho etsa decoloration e hloka metsotso e 90 feela.
3.Kutlo e phahameng le mehlala e fokolang e lokelang ho sebelisoa.E hloka feela 2 μL serum ho serum protein electrophoresis.
4.Wide tshebediso.Tse ling tsa protheine ha ho bonolo ho arola pampiri electrophoresis, joaloka Alpha-fetoprotein, bacteriolytic enzyme, insulin, le histone joalo-joalo Empa ho molemo ho feta ho sebelisa selulose acetate lera electrophoresis ho ba arola.
5.Easy ho boloka le quantification.Ka mor'a ho daea selulose ea acetate membrane electrophoresis disampole, qoelisa lisampole ka har'a motsoako o motsoako oa glacial acetic acid le ethanol ho etsa poleiti e omeletseng e hlakileng e bolokehang nako e telele.
Hobane cellulose acetate membrane electrophoresis e bonolo ho sebetsa, hona joale e sebelisoa haholo ho hlahloba le ho hlahloba liprotheine tsa plasma, lipoprotein, glycoprotein, Alpha-fetoprotein, enzyme, polypeptide, nucleic acid le biomacromolecule tse ling.
Ho itokisetsa Cellulose Acetate Membrane Electrophoresis
Thepa e sebelisoang e kenyelletsa phepelo ea motlakase e tlase, tanka ea electrophoresis e otlolohileng.Mecha ea litaba e tšehetsang ke cellulose acetate membrane.
Li-reagents:
1.pH8.6 barbitol buffer tharollo: Diethyl barbituric acid, Diethyl sodium pentobarbital;
2.Stain:Ponceau S, Trichloroacetic;
3.TBS / T kapa PBS / T: 95% ethanol, glacial acetic acid;
4.Cleaning Tharollo: anhydrous ethanol, glacial acetic acid.
Beijing Liuyi Biotechnology Co., ltd e na le sistimi e holileng ea cellulose acetate membrane electrophoresis.Moetso oa DYCP-38C ke kamore e boreleli ea pampiri le li-slide electrophoresis, le cellulose acetate membrane electrophoresis.'Mele o ka sehloohong oa bōptjoa, ha ho na leakage phenomenon.Moetso oa phepelo ea motlakase DYY-2C, DYY-6C, DYY-6D, DYY-8C , DYY-10C le DYY-12 o ka fana ka matla bakeng sa DYCP-38C.Ka kakaretso, moreki o rata ho khetha mofuta oa DYY-6C joalo ka phepelo ea motlakase bakeng sa DYCP-38C.
E le sehlahisoa se hlokahalang bakeng sa tanka ea electrophoresis ea DYCP-38C, Liuyi e boetse e fana ka lera la cellulose acetate.Ho na le mefuta e meraro e tloaelehileng ea lera, 7 * 9cm, 2 * 8cm le 12 * 8cm, 'me re ka fana ka boholo bo ikhethileng ba cellulose acetate membrane joalo ka litlhoko tsa hau.
Lintlha mabapi le liteko:
1.Laola mohlala ka lehlakoreng le thata: 1-2UL e loketse
2.Elektrophoresis e sa feleng: matla a hajoale 0.4 ~ 0.6m A/cm
3.pH8.6 barbitol buffer tharollo: Ionic matla 0.06
4.Nako ea ho daea: Metsotso e 5 e lekane
5.Tlhokomelo: beha 'mapa o omileng oa electrophoresis ka tharollo ea ho hloekisa bakeng sa metsotso ea 10-15, ebe o e ntša ebe o e khomarela ka khalase e hloekileng, ka mor'a hore e ome, e tla fetoha' mapa oa filimi o hlakileng.
Letšoao la Liuyi le na le nalane ea lilemo tse fetang 50 Chaena mme k'hamphani e ka fana ka lihlahisoa tse tsitsitseng le tsa boleng bo holimo lefats'eng lohle.Nts'etsopele ea lilemo, e tšoaneloa ke khetho ea hau!
Ho fumana lintlha tse ling ka rona, ka kopo ikopanye le rona ka lengolo-tsoibila[imeile e sirelelitsoeng], [imeile e sirelelitsoeng].
Nako ea poso: Apr-01-2022