Sistimi ea semi-dry blotting ea DYCP-40C e sebelisoa hammoho le phepelo ea motlakase ea electrophoresis bakeng sa ho fetisetsa molek'hule ea protheine ka potlako ho tloha gel ho ea ho membrane joalo ka membrane ea nitrocellulose. Semi-dry blotting e etsoa ka li-electrode tsa graphite plate ka tlhophiso e tšekaletseng, e kopanya gel le lera lipakeng tsa maqephe a pampiri e sefang e kolobisitsoeng e sebetsang joalo ka polokelo ea ion. Nakong ea phetisetso ea electrophoretic, limolek'hule tse qosoang hampe li falla ka ntle ho gel ebe li ea ho electrode e ntle, moo li kenngoa ka lera. Li-electrode tsa poleiti, tse arohaneng feela ke li-gel le li-filter stack, li fana ka matla a maholo a tšimo (V / cm) ho pholletsa le gel, ho etsa phetiso e sebetsang haholo, e potlakileng.