Tlhaloso ea Tanka ea Electrophoresis | |
Boholo ba Gel (LxW) | 83 × 73 limilimithara |
Kamo | Liliba tse 10 (Tlwaelehileng) Liliba tse 15 (Ha ho boikhethelo) |
Botenya ba Kamano | 1.0 mm (E Tlwaelehileng) 0.75, 1.5 mm (Kgetho) |
Letlapa le Lekhutšoane la Khalase | 101 × 73 limilimithara |
Letlapa la Glass la Spacer | 101 × 82 limilimithara |
Bolumo ea Buffer | 300 ml |
Litlhaloso tsa Transfer Module | |
Sebaka sa Blotting (LxW) | 100 × 75 limilimithara |
Palo ea Li-Gel Holders | 2 |
Bohole ba Electrode | 4cm |
Bolumo ea Buffer | 1200ml |
Tlhaloso ea Electrophoresis Power Supply | |
Boemo (LxWxH) | 315 x 290 x 128mm |
Voltage e hlahisoang | 6-600V |
Output Current | 4-400mA |
Matla a Output | 240W |
Sephetho sa Output | Lipara tse 4 ka ho latellana |
Mokhoa oa ho fetisa electrophoresis ka mokhoa o le mong o na le tanka ea electrophoresis e nang le sekwahelo, phepelo ea matla e nang le phanele ea taolo, le module ea phetisetso e nang le li-electrode. Tanka ea electrophoresis e sebelisoa ho lahlela le ho tsamaisa li-gel, 'me mochine oa ho fetisa o sebelisetsoa ho tšoara sandwich ea gel le membrane nakong ea ts'ebetso ea phetisetso,' me e na le lebokose le pholileng ho thibela ho futhumala. Motlakase o fana ka motlakase o hlokahalang ho tsamaisa gel le ho khanna phetisetso ea limolek'hule ho tloha gel ho ea ho lera, 'me e na le sebaka sa ho laola se sebetsang hantle bakeng sa ho beha electrophoresis le maemo a ho fetisa. Mojule oa phetisetso o kenyelletsa li-electrode tse behiloeng ka tanka ebe li kopana le gel le membrane, li tlatsa potoloho ea motlakase e hlokahalang bakeng sa ho fetisoa.
The electrophoresis fetisa tsamaiso ea tsohle-in-one ke sesebelisoa sa bohlokoa bakeng sa bafuputsi le litsebi tse sebetsang ka lisampole tsa protheine. Moralo oa eona o kopaneng le boiketlo ba tšebeliso e e etsa tlatsetso ea bohlokoa ho laboratoring efe kapa efe e amehang ho baeloji ea limolek'hule kapa lipatlisiso tsa biochemistry.
The electrophoresis transfer all-in-one system ke sesebelisoa sa bohlokoa lefapheng la biology ea limolek'hule, haholo-holo tlhahlobo ea protheine. Ka mor'a moo, liprotheine tse fetisitsoeng li fumanoa li sebelisa li-antibodies tse itseng ka mokhoa o bitsoang Western blotting. Mokhoa ona o lumella bafuputsi ho khetholla liprotheine tse itseng tse khahlang le ho lekanya maemo a bona a polelo.
• Sehlahisoae lekana bakeng sa boholo bo nyane LEQEPHE gel electrophoresis;
•Sehlahisoa's, lisebelisoa li lumellana ka botlalo le lihlahisoa tsa brand tse ka sehloohong 'marakeng;
•Sebopeho se tsoetseng pele le moralo o bonolo;
• Etsa bonnete ba hore ho na le phello e ntle ea liteko ho tloha ho gel casting ho ea ho gel e sebetsang;
•Fetisetsa ka potlako li-gel tse nyane tsa boholo;
•Likasete tse peli tsa Gel holder li ka kenngoa ka tankeng;
•E ka sebelisa li-gel tse 2 ka hora e le 'ngoe. E ka sebetsa bosiu bohle bakeng sa phetisetso e tlase ea matla;
•Likhasete tsa li-gel tse nang le mebala e fapaneng li netefatsa hore li behiloe hantle.
P: Electrophoresis transfer all-in-one system e sebelisetsoa eng?
A: The electrophoresis fetisa tsamaiso ea tsohle-in-one e sebelisoa bakeng sa ho fetisetsa liprotheine ho tloha polyacrylamide gel holim'a lera bakeng sa tlhahlobo e eketsehileng, joalo ka Western blotting.
P: Ke boholo bofe ba gel e ka etsoang le ho fetisoa ka ho sebelisa electrophoresis ho fetisa tsamaiso eohle-in-one?
A: electrophoresis fetisa tsamaiso eohle-in-one ka lahlela le matha gel boholo 83X73cm bakeng sa ho lahla letsoho, le 86X68cm pele ho lahla gel. Sebaka sa phetisetso ke 100X75cm.
P: Electrophoresis e fetisetsa tsamaiso eohle e le 'ngoe e sebetsa joang?
A: The electrophoresis fetisa tsamaiso eohle-in-one sebelisa electrophoresis ho fetisetsa liprotheine ho tloha gel ho lera. Liprotheine li qala ho aroloa ka boholo ho sebelisa polyacrylamide gel electrophoresis (PAGE) ebe li fetisetsoa lera ho sebelisa tšimo ea motlakase.
P: Ke li-membrane tsa mofuta ofe tse ka sebelisoang ka mokhoa oa ho fetisa electrophoresis tsohle-in-one?
A: Mefuta e fapaneng ea li-membrane e ka sebelisoa ka electrophoresis phetisetso ea tsohle-in-one tsamaiso ea nitrocellulose le PVDF (polyvinylidene difluoride) membranes.
P: Na electrophoresis e ka fetisa tsamaiso eohle-in-one e ka sebelisoa bakeng sa tlhahlobo ea DNA?
A: Che, The electrophoresis transfer system all-in-one e etselitsoe ka ho khetheha bakeng sa tlhahlobo ea protheine 'me e ke ke ea sebelisoa bakeng sa tlhahlobo ea DNA.
Q: Melemo ea ho sebelisa electrophoresis fetisa tsamaiso eohle-in-one ke efe?
A: Mokhoa oa ho fetisa electrophoresis ka mokhoa o le mong o lumella ho fetisetsa liprotheine ka katleho ho tloha ho gel ho ea ho lera, ho fana ka kutloisiso e phahameng le ho khetheha ha ho fumanoa ha protheine. Hape ke sistimi e bonolo ea tsohle-in-one e nolofatsang ts'ebetso ea ho hlakola Bophirimela.
P: Electrophoresis e fetisetsa tsamaiso ea bohle-in-one e lokela ho bolokoa joang?
A: The electrophoresis fetisa tsamaiso ea tsohle-in-one e hloekisoe ka mor'a hore e mong le e mong e sebelisoe ebe e bolokoa sebakeng se hloekileng, se omileng. Li-electrode le likarolo tse ling li lokela ho hlahlojoa khafetsa bakeng sa tšenyo leha e le efe kapa ho senyeha.