Pulsed Field Gel Electrophoresis CHEF Mapper A1
Litlhaloso
| Mohlala | MOPHEHI 'Mapa A1 |
| Voltage Gradient | 0.5V/cm ho isa ho 9.6V/cm, e eketsoa ka 0.1V/cm |
| Boholo ba Hona Joale | 0.5A |
| Maximum Voltage | 350V |
| Pulse Angle | ±120° |
| Nako Gradient | Linear |
| Ho Fetola Nako | 50ms ho isa ho 18h |
| Nako e Phahameng ea ho Matha | 999h |
| Palo ea li-Elektrode | 24, e laoloang ka boikemelo |
| Mocheso Range | 0 ℃ ho 50 ℃, phoso ea ho lemoha <± 0.5 ℃ |
Tlhaloso
Pulsed field gel electrophoresis (PFGE) e arola limolek'hule tsa DNA ka ho fapanyetsana sebaka sa motlakase pakeng tsa lipara tse fapaneng tsa li-electrode tse shebaneng le sebaka, e leng se bakang limolek'hule tsa DNA, tse ka bang limilione tsa lipara tsa motheo tse telele ho khutlela morao le ho falla ka li-pores tsa agarose gel ka lebelo le fapaneng. E finyella qeto e phahameng ka har'a mefuta ena 'me e sebelisoa haholo-holo ho baeloji ea maiketsetso; ho tsebahatsa leloko la baeloji le likokoana-hloko; ho etsa lipatlisiso ka lefu la limolek'hule; lithuto tsa likaroloana tse kholo tsa plasmid; sebakeng sa liphatsa tsa lefutso tsa mafu; 'mapa oa' mele oa liphatsa tsa lefutso, tlhahlobo ea RFLP, le khatiso ea menoana ea DNA; lipatlisiso tsa lefu la lisele tse hlophisitsoeng; lithuto tsa tšenyo le tokiso ea DNA; ho itšehla thajana le tlhahlobo ea DNA ea genomic; ho arohana ha chromosomal DNA; kaho, boitsebahatso, le tlhahlobo ea lilaebrari tsa likarolo tse kholo tsa genomic; le transgenic research.t concentrations e tlase joalo ka 0.5 ng/µL (dsDNA).
Kopo
E loketse ho lemoha le ho arola limolek'hule tsa DNA ho tloha ho 100bp ho ea ho 10Mb ka boholo, ho finyella qeto e phahameng ka har'a mefuta ena.
Sebopeho
• Theknoloji e Tsoetseng Pele: E kopanya CHEF le PACE pulsed-field technologies ho finyella liphello tse ntle ka litsela tse otlolohileng, tse sa kobehang.
• Taolo e Ikemetseng: E na le li-electrode tsa platinum tse 24 tse laoloang ka boikemelo (bophara ba 0.5mm), 'me e mong le e mong o ka nkeloa sebaka ka bomong.
• Mosebetsi o Ikemetseng oa ho Balla: E kopanya mefuta e mengata ea lintho tse ka sehloohong tse kang voltage gradient, mocheso, switjha angle, nako ea pele, nako ea ho qetela, nako ea ho fetoha ha hona joale, kakaretso ea nako ea ho sebetsa, motlakase, le hona joale bakeng sa lipalo tsa othomathike, e thusa basebelisi ho fihlela maemo a nepahetseng a liteko.
• Algorithm e Ikhethang: E sebelisa algorithm e ikhethang ea taolo ea pulse bakeng sa litlamorao tse ntle tsa karohano, e khethollang habonolo lipakeng tsa DNA ea mohala le selikalikoe, ka karohano e ntlafalitsoeng ea DNA e chitja e kholo.
• Boiketsetso: E rekota le ho qala hape electrophoresis haeba sistimi e sitisoa ka lebaka la ho tima matla.
• Mosebelisi-Configuable: E lumella basebelisi ho ipehela maemo a bona.
• Flexibility: Sistimi e ka khetha li-voltage tse khethehileng le linako tsa ho switjha bakeng sa mefuta e itseng ea boholo ba DNA.
• Skrine se Seholo: E na le skrine ea LCD ea 7-inch bakeng sa ts'ebetso e bonolo, e nang le taolo e ikhethang ea software bakeng sa tšebeliso e bonolo le e bonolo.
• Ho lemoha Mocheso: Lisebelisoa tse peli tsa mocheso li lemoha mocheso oa buffer ka ho toba ka moeli oa phoso o ka tlaase ho ±0.5℃.
• Sistimi ea Potoloho: E tla le sistimi ea potoloho ea buffer e laolang ka nepo le ho hlokomela mocheso oa tharollo ea buffer, e netefatsang mocheso o sa feleng le ho leka-lekana ha ionic nakong ea electrophoresis.
• Tšireletseho e Phahameng: E kenyelletsa sekoaelo sa tšireletso sa acrylic se bonaletsang se itšehang motlakase ha se phahamisoa, hammoho le ts'ebetso ea ts'ireletso e feteletseng le e se nang moroalo.
• Boemo bo Fetohang: Tanka ea electrophoresis le gel caster e na le maoto a feto-fetohang bakeng sa ho lekana.
• Moqapi oa Mold: Tanka ea electrophoresis e entsoe ka mohaho o kopantsoeng oa hlobo ntle le tlamahano; rack ea electrode e na le li-electrode tsa platinum tsa 0.5mm, tse netefatsang ho tšoarella le liphello tse tsitsitseng tsa liteko.
LBH
P: Pulsed Field Gel Electrophoresis ke eng?
A: Pulsed Field Gel Electrophoresis ke mokhoa o sebelisoang ho arola limolek'hule tse kholo tsa DNA ho latela boholo ba tsona. E kenyelletsa ho fapanyetsana tsela ea sebaka sa motlakase ka har'a matrix ea gel ho nolofalletsa ho arola likaroloana tsa DNA tse kholo haholo hore li ka rarolloa ka tloaelo ea agarose gel electrophoresis.
Q: Ke litšebeliso life tsa Pulsed Field Gel Electrophoresis?
A: Pulsed Field Gel Electrophoresis e sebelisoa haholo ho baeloji ea limolek'hule le liphatsa tsa lefutso bakeng sa:
Ho etsa 'mapa oa limolek'hule tse kholo tsa DNA, tse kang li-chromosome le li-plasmids.
• Ho khetha boholo ba genome.
• Ho ithuta ho fapana ha liphatsa tsa lefutso le likamano tsa ho iphetola ha lintho.
• Epidemiology ea limolek'hule, haholo-holo bakeng sa ho latela ho qhoma ha mafu a tšoaetsanoang.
• Tlhahlobo ea tšenyo le tokiso ea DNA.
• Ho fumana boteng ba liphatsa tsa lefutso tse itseng kapa tatelano ea DNA.
P: Pulsed Field Gel Electrophoresis e sebetsa joang?
A: Pulsed Field Gel Electrophoresis e sebetsa ka ho beha limolek'hule tsa DNA sebakeng sa motlakase sa pulsed se chenchana ka tsela. Sena se lumella limolek'hule tse kholo tsa DNA hore li ikamahanye hape pakeng tsa maqhubu, e leng ho nolofalletsang ho tsamaea ha tsona ka matrix a gel. Limolek'hule tse nyenyane tsa DNA li tsamaea ka potlako ka har'a gel, ha tse kholoanyane li tsamaea butle, li lumella ho arohana ha tsona ho latela boholo.
P: Molao-motheo oa Pulsed Field Gel Electrophoresis ke ofe?
A: Pulsed Field Gel Electrophoresis e arola limolek'hule tsa DNA ho latela boholo ba tsona ka ho laola nako le tataiso ea maqhubu a tšimo ea motlakase. Tšimo e fapanyetsanoang e etsa hore limolek'hule tse kholo tsa DNA li tsoele pele ho itlhophisa, e leng se lebisang ho falleng ha tsona ka matrix a gel le ho arohana ho ea ka boholo.
P: Melemo ea Pulsed Field Gel Electrophoresis ke efe?
A: Qeto e phahameng bakeng sa ho arola limolek'hule tse kholo tsa DNA ho fihla ho li-base pairs tse limilione tse 'maloa.Bokhoni ba ho rarolla le ho khetholla likaroloana tsa DNA tsa boholo bo tšoanang.Versatility ka tšebeliso, ho tloha ho mofuta oa likokoana-hloko ho ea ho liphatsa tsa lefutso le liphatsa tsa lefutso.Mokhoa o thehiloeng bakeng sa lithuto tsa lefu la seoa le 'mapa oa lefutso.
P: Ke lisebelisoa life tse hlokahalang bakeng sa Pulsed Field Gel Electrophoresis?
A: Pulsed Field Gel Electrophoresis hangata e hloka lisebelisoa tsa electrophoresis tse nang le li-electrode tse khethehileng bakeng sa ho hlahisa masimo a pulsed. Agarose gel matrix e nang le mahloriso a loketseng le buffer. Phepelo ea matla e khonang ho hlahisa li-pulses tse matla haholo.Sistimi ea ho pholisa ho qhala mocheso o hlahisoang nakong ea electrophoresis, le pompo ea potoloho.
