PCR Thermal Cycler WD-9402M
Litlhaloso
| Mohlala | WD-9402M |
| Bokhoni | 96×0.2ml |
| Tube | 96x0.2ml (PCR poleiti ntle le / semi skirt), 12x8x0.2ml e hlobolisa, 8x12x0.2ml e hlobolisa, 0.2ml li-tubes (bophahamo ba 20 ~ 23mm) |
| Thibela Mocheso Range | 0-105 ℃ |
| Thibela ho nepahala ha Mocheso | ±0.2℃ |
| Thibela Mocheso o tšoanang | ±0.5℃ |
| Sekhahla sa ho futhumatsa (kakaretso) | 4℃ |
| Sekhahla sa ho Pholisa (karolelano) | 3℃ |
| Taolo ea Mocheso | Thibela/Tube |
| Mocheso oa Gradient. Range | 30-105 ℃ |
| Sekhahla sa ho futhumatsa | 5℃/s |
| Max.Sekhahla sa Pholiso 4.5℃ /S | 4.5℃/s |
| Gradient Set Span | Max. 42℃ |
| Ho Nepaha ha Mocheso oa Gradient | ±0.3℃ |
| Mocheso o nepahetseng oa pontšo | 0.1℃ |
| Mocheso oa Sekoaelo sa Mocheso o fapaneng | 30 ℃ ~ 110 ℃ |
| Sekoaelo sa ho futhumatsa ka ho iketsa | Koala ka bohona ha sampole e ka tlase ho 30 ℃ kapa lenaneo le felile |
| Nako ea eketseha / ea fokotseha | -599~599 S bakeng sa PCR e telele |
| Mocheso oa eketseha / oa fokotseha | -9.9 ~ 9.9℃ bakeng sa Touchdown PCR |
| Sebali sa nako | 1s~59min59sec/ E sa feleng |
| Mananeo a bolokiloeng | 10000+ |
| Max. Lipotoloho | 99 |
| Max.Mehato | 30 |
| Emisa Mosebetsi | Ee |
| Mosebetsi oa Touchdown | Ee |
| Mosebetsi o molelele oa PCR | Ee |
| Puo | Senyesemane |
| Mosebetsi oa ho emisa lenaneo | Ee |
| 16℃ Temperature Holding Function | E sa feleng |
| Boemo ba ts'ebetso ea nako ea sebele | Mongolo oa setšoantšo o hlahisitsoe |
| Puisano | USB 2.0 |
| Litekanyo | 200mm×300mm×170mm (W×D×H) |
| Boima ba 'mele | 4.5kg |
| Phepelo ea motlakase | 100-240VAC , 50/60Hz, 600W |
Tlhaloso
Thermal cycler e sebetsa ka ho futhumatsa khafetsa le ho pholisa motsoako oa karabelo o nang le template ea DNA kapa RNA, primers le nucleotide. Thempereichara ea baesekele e laoloa ka nepo ho fihlela mehato e hlokahalang ea denaturation, annealing, le katoloso ea ts'ebetso ea PCR.
Ka tloaelo, sebaesekele se futhumatsang se na le boloko bo nang le liliba tse ngata kapa li-tubes moo motsoako oa karabelo o behiloeng teng, 'me mocheso selibeng ka seng o laoloa ka boikemelo. Sebaka sena se futhumatsoa 'me se pholile ho sebelisoa ntho ea Peltier kapa tsamaiso e' ngoe ea ho futhumatsa le ho pholisa.
Boholo ba libaesekele tse futhumatsang li na le sebopeho se bonolo sa mosebelisi se lumellang mosebelisi ho hlophisa le ho lokisa liparamente tsa libaesekele, joalo ka mocheso oa annealing, nako ea katoloso, le palo ea lipotoloho. Li kanna tsa ba le ponts'o ho lekola tsoelo-pele ea karabelo, 'me mefuta e meng e ka fana ka likarolo tse tsoetseng pele joalo ka taolo ea mocheso o tlase, litlhophiso tse ngata tsa li-block, le tlhahlobo le taolo e hole.
Kopo
Polymerase Chain Reaction (PCR) ke mokhoa oa baeloji ea limolek'hule o sebelisoang haholo lits'ebetsong tse fapaneng. Lisebelisoa tse ling tse tloaelehileng tsa PCR li kenyelletsa:
DNA Amplification: Sepheo sa mantlha sa PCR ke ho holisa tatelano e itseng ea DNA. Sena se bohlokoa bakeng sa ho fumana litekanyo tse lekaneng tsa DNA bakeng sa litlhahlobo kapa liteko tse ling.
Teko ea Liphatsa tsa lefutso: PCR e sebelisoa haholo tlhahlobong ea liphatsa tsa lefutso ho fumana matšoao a itseng a lefutso kapa liphetoho tse amanang le mafu. E bohlokoa molemong oa tlhahlobo le ho ithuta ka liphatsa tsa lefutso.
DNA Cloning: PCR e sebelisoa ho hlahisa palo e kholo ea sekhechana se itseng sa DNA, se ka fetoloang hore e be vector bakeng sa ho qhekella kapa tlhahlobo.
Tlhahlobo ea DNA ea Forensic: PCR e bohlokoa ho mahlale a forensic bakeng sa ho holisa lisampole tsa DNA tse fumanoeng litsing tsa botlokotsebe. E thusa ho khetholla batho ka bomong le ho theha likamano tsa lefutso.
Ho fumanoa ha Microbial: PCR e sebelisoa bakeng sa ho lemoha likokoana-hloko tsa likokoana-hloko lisampoleng tsa bongaka kapa disampole tsa tikoloho. E lumella ho tsebahatsa ka potlako mahlahana a tšoaetsanoang.
Quantitative PCR (qPCR kapa PCR ea Nako ea 'Nete): qPCR e nolofalletsa quantification ea DNA nakong ea ts'ebetso ea ho holisa. E sebelisoa ho lekanya maemo a polelo ea liphatsa tsa lefutso, ho bona bongata ba vaerase, le ho lekanya palo ea tatelano e itseng ea DNA.
Lithuto tsa Phetoho ea Limolek'hule: PCR e sebelisoa lithutong tse hlahlobang phapang ea liphatsa tsa lefutso ka har'a baahi, likamano tsa ho iphetola ha lintho, le tlhahlobo ea phylogenetic.
Tlhahlobo ea DNA (eDNA) ea Tikoloho: PCR e sebelisoa ho bona boteng ba lintho tse phelang tse itseng lisampong tsa tikoloho, tse kenyang letsoho lithutong tsa mefuta-futa ea lintho tse phelang le tikoloho.
Boenjiniere ba Liphatsa tsa lefutso: PCR ke sesebelisoa sa bohlokoa ho boenjineri ba liphatsa tsa lefutso ho hlahisa tatelano e khethehileng ea DNA linthong tse phelang. E sebelisoa ha ho etsoa liphatsa tsa lefutso (GMOs).
Ho hlophisa Tokiso ea Laebrari: PCR e nka karolo ho lokiseng lilaeborari tsa DNA bakeng sa mahlale a tatellano a moloko o latelang. E thusa ho holisa likhechana tsa DNA bakeng sa lits'ebetso tsa tatellano e tlase.
Mutagenesis-Directed Site: PCR e sebelisoa bakeng sa ho hlahisa liphetoho tse itseng ka tatellano ea DNA, ho lumella bafuputsi ho ithuta liphello tsa liphetoho tse itseng tsa lefutso.
DNA Fingerprinting: PCR e sebelisoa mekhoeng ea ho hatisa menoana ea DNA bakeng sa boitsebahatso ba motho ka mong, tlhahlobo ea bontate, le ho theha likamano tsa tlhaho.
Sebopeho
• Ponahalo e ntle, boholo ba compact, le sebopeho se thata.
• E na le fene e sebetsang hantle, e khutsitseng ea axial-flow bakeng sa ts'ebetso e khutsitseng ea ts'ebetso.
•E fana ka ts'ebetso e pharalletseng ea 30℃, e lumellang ntlafatso ea maemo a liteko ho fihlela litlhoko tse matla tsa liteko.
•5-inch e phahameng-definition color-definition screenscreen for the intuitive and easy operation, e nolofalletsang ho hlophisa, ho boloka, le ho tsamaisa mananeo ha bonolo.
• Sistimi ea tšebetso ea boemo ba indasteri, e tsamaisang ts'ebetso e tsoelang pele le e se nang liphoso 7x24.
• Ho fetisoa ha data ka potlako ho USB flash drive bakeng sa bekapo e bonolo ea lenaneo, ho matlafatsa bokhoni ba polokelo ea data.
• Theknoloji e tsoetseng pele ea ho futhumatsa semiconductor le thekenoloji e ikhethang ea ho laola mocheso oa PID e phahamisa ts'ebetso ka kakaretso ho ea libakeng tse ncha: ho nepahala ho laola mocheso o phahameng, ho futhumala ka potlako le litekanyetso tsa ho pholisa, le mocheso oa module o ajoang ka mokhoa o tšoanang.
LBH
P: Sebaesekele se futhumatsang ke eng?
A: Thermal cycler ke sesebelisoa sa laboratori se sebelisetsoang ho holisa DNA kapa RNA tatelano ka polymerase chain reaction (PCR). E sebetsa ka ho palama libaesekele ho pholletsa le letoto la liphetoho tsa mocheso, ho lumella tatellano e khethehileng ea DNA hore e atolosoe.
P: Likarolo tsa mantlha tsa sebaesekele se futhumatsang ke life?
A: Likarolo tse ka sehloohong tsa sebaesekele se futhumatsang li kenyelletsa block block, thermoelectric cooler, sensors mocheso, microprocessor, le phanele ea taolo.
P: Sebaesekele se futhumatsang se sebetsa joang?
K: Sebaesekele se futhumatsang se sebetsa ka ho futhumatsa le ho pholisa lisampole tsa DNA letotong la lipotoloho tsa mocheso. Mokhoa oa ho palama baesekele o kenyelletsa mekhahlelo ea denaturation, annealing, le katoloso, e 'ngoe le e 'ngoe e na le mocheso le nako e itseng. Lipotoloho tsena li lumella tatellano e khethehileng ea DNA hore e holisoe ka polymerase chain reaction (PCR).
P: Ke lintlha life tsa bohlokoa tse lokelang ho nahanoa ha u khetha sebaesekele se futhumatsang?
K: Lintlha tse ling tsa bohlokoa tse lokelang ho nahanoa ha u khetha sebaesekele se futhumatsang li kenyelletsa palo ea liliba kapa li-tubes tsa reaction, sebaka sa mocheso le lebelo la tsela, ho nepahala le ho tšoana ha taolo ea mocheso, le sebopeho sa mosebelisi le bokhoni ba software.
P: U boloka sebaesekele se futhumatsang joang?
K: Ho boloka sebaesekele se futhumatsang mocheso, ho bohlokoa ho hloekisa sebaka sa ho futhumatsa le li-tubes khafetsa, ho hlahloba hore na ha ho na lintho tse senyehileng, le ho lekanya li-sensor tsa mocheso ho netefatsa taolo e nepahetseng le e tsitsitseng ea mocheso. Hape ke habohlokoa ho latela litaelo tsa moetsi bakeng sa tlhokomelo ea kamehla le ho lokisa.
P: Ke mehato efe e tloaelehileng ea ho rarolla mathata bakeng sa sebaesekele se futhumatsang?
K: Mehato e meng e tloaelehileng ea ho rarolla mathata bakeng sa sebaesekele se futhumatsang e kenyelletsa ho hlahloba likarolo tse hlephileng kapa tse senyehileng, ho netefatsa mocheso o nepahetseng le maemo a nako, le ho lekola li-tubes kapa lipoleiti bakeng sa tšilafalo kapa tšenyo. Ho bohlokoa hape ho bua ka litaelo tsa moetsi bakeng sa mehato e tobileng ea ho rarolla mathata le tharollo.
