PCR Thermal Cycler WD-9402D
Litlhaloso
| Mohlala | WD-9402D |
| Bokhoni | 96×0.2ml |
| Tube | 0.2ml tube, 8 strips, Half skirt96 liliba poleiti, Ha ho moleka 96 liliba poleiti |
| Bolumo ea Karabelo | 5-100ul |
| Mocheso Range | 0-105 ℃ |
| MAX. Ramp Rate | 5℃/s |
| Ho tšoana | ≤±0.2℃ |
| Ho nepahala | ≤±0.1℃ |
| Qeto ea Pontšo | 0.1℃ |
| Taolo ea Mocheso | Thibela/Tube |
| Ramping Rate e ka fetoloa | 0.01-5 ℃ |
| Mocheso oa Gradient. Range | 30-105 ℃ |
| Mofuta oa Gradient | Tloaelehileng Gradient |
| Ho ata ha Gradient | 1-42 ℃ |
| Mocheso oa Sekoahelo o Chesang | 30-115 ℃ |
| Palo ea Mananeo | 20000 +(USB FLASH) |
| Max. No. ea Mohato | 40 |
| Max. No. ea Cycle | 200 |
| Keketso ea Nako / Phokotso | 1 Sec - 600 Sec |
| Keketso ea Mocheso / Phokotso | 0.1-10.0℃ |
| Emisa Mosebetsi | Ee |
| Tšireletso ea Boitsebiso ba Auto | Ee |
| Tšoara ho 4 ℃ | Ka ho sa feleng |
| Mosebetsi oa Touchdown | Ee |
| Mosebetsi o molelele oa PCR | Ee |
| Puo | Senyesemane |
| Khomphuta Software | Ee |
| Mohala oa mohala oa APP | Ee |
| LCD | 10.1 inch, 1280 × 800 pel'a |
| Puisano | USB2.0, WIFI |
| Litekanyo | 385mm×270mm×255mm (L×W×H) |
| Boima ba 'mele | 10kg |
| Phepelo ea motlakase | 100-240VAC , 50/60Hz , 600 W |
Tlhaloso
Thermal cycler e sebetsa ka ho futhumatsa khafetsa le ho pholisa motsoako oa karabelo o nang le template ea DNA kapa RNA, primers le nucleotide. Thempereichara ea baesekele e laoloa ka nepo ho fihlela mehato e hlokahalang ea denaturation, annealing, le katoloso ea ts'ebetso ea PCR.
Ka tloaelo, sebaesekele se futhumatsang se na le boloko bo nang le liliba tse ngata kapa li-tubes moo motsoako oa karabelo o behiloeng teng, 'me mocheso selibeng ka seng o laoloa ka boikemelo. Sebaka sena se futhumatsoa 'me se pholile ho sebelisoa ntho ea Peltier kapa tsamaiso e' ngoe ea ho futhumatsa le ho pholisa.
Boholo ba libaesekele tse futhumatsang li na le sebopeho se bonolo sa mosebelisi se lumellang mosebelisi ho hlophisa le ho lokisa liparamente tsa libaesekele, joalo ka mocheso oa annealing, nako ea katoloso, le palo ea lipotoloho. Li kanna tsa ba le ponts'o ho lekola tsoelo-pele ea karabelo, 'me mefuta e meng e ka fana ka likarolo tse tsoetseng pele joalo ka taolo ea mocheso o tlase, litlhophiso tse ngata tsa li-block, le tlhahlobo le taolo e hole.
Kopo
Cloning ea Genome; Ho lokisoa ha PCR ea asymmetric ea DNA e le 'ngoe bakeng sa tatellano ea DNA; PCR e fapaneng bakeng sa qeto ea libaka tse sa tsejoeng tsa DNA; Phetolelo ea morao-rao ea PCR (RT-PCR). Bakeng sa ho lemoha boemo ba liphatsa tsa lefutso liseleng, le palo ea kokoana-hloko ea RNA le ho kopanya ka ho toba cDNA ka liphatsa tsa lefutso tse itseng; ho holisa ka potlako lipheletsong tsa cDNA; ho fumanoa ha liphatsa tsa lefutso; e ka sebelisoa ho lemoha mafu a baktheria le a kokoana-hloko; ho hlahlojoa ha mafu a lefutso; ho hlahlojoa ha lihlahala; lipatlisiso tsa bongaka joalo ka bopaki ba 'mele, li ke ke tsa sebelisoa lipatlisisong tsa bongaka tsa bongaka.
Featured
• Sekhahla se phahameng sa ho futhumatsa le ho pholisa, max. Sekhahla sa lebelo la 8 ℃/s;
• Ho qala bocha ka boiketsetso ka mor'a ho hloleha ha matla. Ha matla a tsosolositsoeng a ka tsoela pele ho tsamaisa lenaneo le sa phethoang;
• Tobetso e le 'ngoe e potlakileng ea ho incubation e ka khotsofatsa litlhoko tsa liteko tse kang denaturation, enzyme cutting/enzyme-link le ELISA;
• Mocheso o chesang oa sekwahelo le mokhoa oa ho sebetsa oa sekoahelo se chesang o ka hlophisoa ho kopana le litlhoko tse fapaneng tsa liteko;
• E sebelisa li-module tsa Peltier tsa bophelo bo bolelele ba libaesekele;
• Anodized aluminium module e nang le matlafatso ea boenjiniere, e bolokang ts'ebetso ea ho tsamaisa mocheso ka potlako 'me e na le khanyetso e lekaneng ea kutu;
• Likhahla tse potlakileng tsa mocheso, tse nang le sekhahla se phahameng sa 5°C/s, ho boloka nako ea bohlokoa ea liteko;
• Adaptive pressure bar-style sekoahelo sa mocheso, se ka koaloang ka thata ka mohato o le mong mme se ka ikamahanya le bophahamo bo fapaneng ba li-tube;
• Moralo oa moea o ka pele ho ea morao, o lumellang mechini hore e behoe ka lehlakore;
• E sebelisa sistimi ea ts'ebetso ea Android, e tsamaellanang le skrini sa 10.1-inch capacitive touch, se nang le sebopeho sa "graphical menu-style navigation interface, se etsang hore ts'ebetso e be bonolo haholo;
• A haha-ka-ka 11 maemo a tloaelehileng faele dithempleite tsa lenaneo, tse ka ka potlako fetola difaele tse hlokahalang;
• Pontšo ea nako ea sebele ea tsoelo-pele ea lenaneo le nako e setseng, e tšehetsang lenaneo la bohareng ba sesebelisoa sa PCR;
• Konopo e le 'ngoe e potlakileng ea ho kenya tšebetsong, ho finyella litlhoko tsa liteko tse kang denaturation, enzyme digestion/ligation, le ELISA;
• Mocheso o chesang oa sekoahelo le mokhoa oa ho sebetsa oa sekoahelo se chesang o ka behoa ho finyella litlhoko tse fapaneng tsa liteko;
• Ts'ireletso e ikemetseng ea ho tima motlakase, ho etsa lipotoloho tse sa phethoang ka bo eona ka mor'a hore motlakase o khutlisetsoe, ho netefatsa ts'ebetso e bolokehileng ts'ebetsong eohle ea amplification;
• Sehokelo sa USB se ts'ehetsa ho boloka / ho khutlisa data ea PCR ka USB drive hape e ka sebelisa mouse ea USB ho laola sesebelisoa sa PCR;
• E tshehetsa diapdeite tsa software ka USB le LAN;
• Mojule oa WIFI o hahiloeng, o lumellang khomphuta kapa selefouno ho laola lisebelisoa tse ngata tsa PCR ka nako e le 'ngoe ka khokahanyo ea marang-rang;
• E tšehetsa tsebiso ea lengolo-tsoibila ha lenaneo la liteko le phethiloe.
LBH
P: Sebaesekele se futhumatsang ke eng?
A: Thermal cycler ke sesebelisoa sa laboratori se sebelisetsoang ho holisa DNA kapa RNA tatelano ka polymerase chain reaction (PCR). E sebetsa ka ho palama libaesekele ho pholletsa le letoto la liphetoho tsa mocheso, ho lumella tatellano e khethehileng ea DNA hore e atolosoe.
P: Likarolo tsa mantlha tsa sebaesekele se futhumatsang ke life?
A: Likarolo tse ka sehloohong tsa sebaesekele se futhumatsang li kenyelletsa block block, thermoelectric cooler, sensors mocheso, microprocessor, le phanele ea taolo.
P: Sebaesekele se futhumatsang se sebetsa joang?
K: Sebaesekele se futhumatsang se sebetsa ka ho futhumatsa le ho pholisa lisampole tsa DNA letotong la lipotoloho tsa mocheso. Mokhoa oa ho palama baesekele o kenyelletsa mekhahlelo ea denaturation, annealing, le katoloso, e 'ngoe le e 'ngoe e na le mocheso le nako e itseng. Lipotoloho tsena li lumella tatellano e khethehileng ea DNA hore e holisoe ka polymerase chain reaction (PCR).
P: Ke lintlha life tsa bohlokoa tse lokelang ho nahanoa ha u khetha sebaesekele se futhumatsang? K: Lintlha tse ling tsa bohlokoa tse lokelang ho nahanoa ha u khetha sebaesekele se futhumatsang li kenyelletsa palo ea liliba kapa li-tubes tsa reaction, sebaka sa mocheso le lebelo la tsela, ho nepahala le ho tšoana ha taolo ea mocheso, le sebopeho sa mosebelisi le bokhoni ba software.
P: U boloka sebaesekele se futhumatsang joang?
K: Ho boloka sebaesekele se futhumatsang mocheso, ho bohlokoa ho hloekisa sebaka sa ho futhumatsa le li-tubes khafetsa, ho hlahloba hore na ha ho na lintho tse senyehileng, le ho lekanya li-sensor tsa mocheso ho netefatsa taolo e nepahetseng le e tsitsitseng ea mocheso. Hape ke habohlokoa ho latela litaelo tsa moetsi bakeng sa tlhokomelo ea kamehla le ho lokisa.
P: Ke mehato efe e tloaelehileng ea ho rarolla mathata bakeng sa sebaesekele se futhumatsang?
K: Mehato e meng e tloaelehileng ea ho rarolla mathata bakeng sa sebaesekele se futhumatsang e kenyelletsa ho hlahloba likarolo tse hlephileng kapa tse senyehileng, ho netefatsa mocheso o nepahetseng le maemo a nako, le ho lekola li-tubes kapa lipoleiti bakeng sa tšilafalo kapa tšenyo. Ho bohlokoa hape ho bua ka litaelo tsa moetsi bakeng sa mehato e tobileng ea ho rarolla mathata le tharollo.
