Mokhoa oa ho palama libaesekele ho PCR ke ofe?

Polymerase Chain Reaction (PCR) ke mokhoa oa baeloji ea limolek'hule o sebelisetsoang ho holisa likhechana tse itseng tsa DNA. E ka nkoa e le mokhoa o khethehileng oa ho ikatisa ha DNA ka ntle ho ntho e phelang. Karolo e ka sehloohong ea PCR ke bokhoni ba eona ba ho eketsa haholo palo ea DNA.

Kakaretso ea Polymerase Chain Reaction Cycle

Setšoantšo se tsoang Bioninja

Mokhoa oa ho palama libaesekele ho PCR ke ofe?

PCR e na le mehato e meraro ea mantlha:

1. Denaturation of Template DNA: Thempleite ea DNA e futhumatsoa hoo e ka bang 93°C ka nako e itseng, e leng se etsang hore DNA e likhoele tse peli e arohane ho ba likhoele tse le 'ngoe, e lokisetse hore e tlamehe nakong e tlang.

2. Anealing of Template DNA and Primers: Kamora denaturation, thempereichara e theoleloa ho pota 55°C, e leng ho lumellang li-primers ho tlamahana le tatellano e tlatsetsang ho DNA ea template e nang le thipa e le 'ngoe.

3. Katoloso ea Li-Primers: Ka 72 ° C, DNA template-primer complex e atolosoa ka thuso ea DNA polymerase (joaloka Taq DNA polymerase), e sebelisa dNTP e le li-substrates ho kopanya strand e ncha e tlatsetsang e thehiloeng ho template ea DNA.

Mochini oa Beijing LIUYI PCR

Ka ho pheta-pheta lits'ebetso tsa denaturation, annealing, le atoloso, "li-semi-conservative replication strands" tse ngata li hlahisoa, tse ka sebetsang e le litempele bakeng sa lipotoloho tse latelang. Potoloho e 'ngoe le e 'ngoe e nka metsotso e ka bang 2 ho isa ho e 4, e leng se lumellang hore phatsa ea lefutso e holisoe ka makhetlo a limilione nakong ea lihora tse peli ho isa ho tse 3.

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) e ikhethetse ho etsa lisebelisoa tsa electrophoresis ka lilemo tse fetang 50 ka sehlopha sa rona sa litsebi tsa setsebi le setsi sa R&D. Re na le mohala o tšepahalang le o felletseng oa tlhahiso ho tloha ho moralo ho ea ho tlhahlobo, le ntlo ea polokelo, hammoho le tšehetso ea papatso. Lihlahisoa tsa rona tse ka sehloohong ke Electrophoresis Cell (tanka / kamore), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System joalo-joalo Re boetse re fana ka lisebelisoa tsa lab joaloka PCR sesebelisoa, vortex mixer le centrifuge bakeng sa laboratori.

Haeba u na le moralo oa ho reka lihlahisoa tsa rona, ka kopo u se ke oa qeaqea ho ikopanya le rona. U ka re romella molaetsa ka lengolo-tsoibila[imeile e sirelelitsoeng]kapa[imeile e sirelelitsoeng], kapa ka kopo re letsetse ho +86 15810650221 kapa eketsa Whatsapp +86 15810650221, kapa Wechat: 15810650221.

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