DYCP-40C semi-dry blotting system e sebelisoa hammoho le phepelo ea motlakase ea electrophoresis bakeng sa ho fetisetsa liprotheine tse ka har'a li-gels tsa polyacrylamide holim'a lera le kang la nitrocellulose, lera la nylon le lera la PVDF. Semi-dry blotting e etsoa ka li-electrode tsa graphite plate ka tlhophiso e tšekaletseng, ho kopanya gel le lera lipakeng tsa maqephe a pampiri e sefang e kolobisitsoeng e sebetsang joalo ka polokelo ea ion. Nakong ea phetisetso ea electrophoretic, limolek'hule tse qosoang hampe li falla ka ntle ho gel ebe li ea ho electrode e ntle, moo li kenngoa ka lera. Li-electrode tsa poleiti, tse arohaneng feela ke li-gel le li-filter stack, li fana ka matla a maholo a tšimo (V / cm) ho pholletsa le gel, ho etsa phetiso e sebetsang haholo, e potlakileng. Sebaka sa phetisetso ea sele e nyane ea DYCP - 40C electrophoresis ke 150 × 150 (mm), e loketseng ho fetisetsa li-gel tse tloaelehileng, ho kenyelletsa le tse tsoang seleng ea DYCZ-24DN le DYCZ-24EN electrophoresis.
A re ithute haholoanyane ka ts'ebetso ea sesebelisoa sena sa semi-dry trans blot.
Lisebelisoa, lisebelisoa tsa ho sebetsa DYCP-40C
Phepelo ea motlakase ea Electrophoresis DYY-6C, semi-dry trans blot apparatus DYCP-40C, tharollo ea buffer, le lijana tsa tharollo ea buffer. etc.
Mehato ea Ts'ebetso
1. Beha gel e nang le lipoleiti tsa khalase ka har'a tharollo ea buffer ea phetisetso
2. Lekanya boholo ba gel
3.Lokisetsa likotoana tse 3 tsa pampiri ea filthara ho latela boholo ba gel, 'me boholo ba pampiri ea sefa e lokela ho ba kholo ho feta boholo ba gel; Mona re sebelisa pampiri ea filthara ea Whatman;
4.Beha likotoana tse 3 tsa pampiri e sefang ka har'a tharollo ea buffer butle, 'me u tlohelle pampiri e sefang hore e qoele ka har'a buffer ka botlalo, 'me u qobe ho ba le lipululana tsa moea;
5.Lokisetsa le ho khaola membrane ea nitrocellulose ho latela boholo ba gel le pampiri ea sefa; boholo ba membrane ea nitrocellulose e lokela ho ba kholo ho feta boholo ba gel le pampiri ea sefa;
6.Kenya lera la nitrocellulose ka har'a tharollo ea buffer;
7.Ntša likotoana tse 3 tsa pampiri ea sefa 'me u tlohele tharollo ea buffer e eketsehileng ho fihlela ho se na tharollo ea buffer e theohang lera; ebe o beha pampiri ea sefa tlase ho DYCP-40C;
8.Nka gel ho tloha lipoleiti tsa khalase, ka bonolo hloekisa gel ea stacking, 'me u kenye gel ka har'a tharollo ea buffer;
9.Beha gel pampiring ea filthara, qala ho tloha pheletsong e 'ngoe ea gel ho qoba li-bubble tsa moea;
10.Sebelisa sesebelisoa se nepahetseng ho tlosa lipompong tsa moea lipakeng tsa gel le pampiri ea sefa.
11.Koahela lesela la nitrocellulose holim'a gel, lehlakoreng le thata ho ea ho gel. Ebe u sebelisa sesebelisoa se nepahetseng ho tlosa li-bubble tsa moea pakeng tsa lera le gel. Beha likotoana tse 3 tsa pampiri ea sefa holim'a lera. Ho ntse ho hlokahala ho sebelisa sesebelisoa se nepahetseng ho tlosa li-bubble tsa moea lipakeng tsa pampiri ea sefa le lera.
12.Koahela sekoahelo, 'me u behe li-parameter tsa electrophoresis, nako e sa khaotseng ea 80mA;
13.Electrophoresis e etsoa. Re fumana sephetho ka tsela e latelang;
Beijing Liuyi Biotechnology Co., Ltd e khethehile ho etsa lihlahisoa tsa electrophoresis ka lilemo tse fetang 50. Re lik'hamphani tse tiisitsoeng tsa ISO9001 & ISO13485 'me re sebetsa ka ho khetheha ho hlahiseng litanka tsa electrophoresis, lisebelisoa tsa motlakase, UV transilluminator, le litokomane tsa gel & analysis system, hajoale, re fana ka tšebeletso ea OEM bakeng sa bareki ba rona, hammoho le tšebeletso ea ODM.
Hona joale re batla balekane, OEM le barekisi ba amoheloa.
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Nako ea poso: Apr-04-2023