A na u na le mathata a ho lokisa gel ea agarose?Ha re latelengsetsebi sa rona sa lab ho lokisa gel.
Mokhoa oa ho lokisa gel ea agarose o kenyelletsa mehato e latelang:
Ho lekanya phofo ea Agarose
Beha palo e hlokahalang ea phofo ea agarose ho ea ka mahloriso a lakatsehang bakeng sa teko ea hau. Maemo a tloaelehileng a agarose a tsoa ho 0.5% ho isa ho 3%. Mekhahlelo e phahameng e sebelisoa bakeng sa ho arola limolek'hule tse nyane tsa DNA, athe tse tlase ke tsa limolek'hule tse kholoanyane.
Ho Lokisetsa Tharollo ea Buffer
Eketsa phofo ea agarose ho buffer e loketseng ea electrophoresis, e kang 1× TAE kapa 1× TBE. Bophahamo ba buffer bo lokela ho ts'oana le bophahamo ba gel e hlokahalang bakeng sa teko ea hau.
Ho qhaqha Agarose
Chesa motsoako oa agarose le buffer ho fihlela agarose e qhibiliha ka ho feletseng. Sena se ka etsoa ka microwave kapa sejana se chesang. Hlohlelletsa tharollo ka nakoana ho thibela hore e se ke ea bela. Tharollo ea agarose e lokela ho hlaka ntle le likaroloana tse bonahalang.
Ho pholisa Tharollo ea Agarose
Lumella tharollo ea agarose e futhumetseng hore e pholile ho ea ho 50-60 ° C. Hlohlelletsa tharollo nakong ea ho pholisa ho thibela ho tiea pele ho nako.
Ho kenyelletsa Nucleic Acid Stain (Ho ikhethela)
Haeba u batla ho bona DNA kapa RNA ka gel, u ka eketsa letheba la nucleic acid, joalo ka GelRed kapa ethidium bromide, sethaleng sena. Ha u tšoara matheba ana, roala liatlana 'me u be hlokolosi, kaha li ka ba chefo.
Ho lahla Gel
Tšela tharollo ea agarose e pholileng ka har'a hlobo e lokiselitsoeng ea gel electrophoresis. Kenya kama ho etsa liliba tsa sampole, ho etsa bonnete ba hore kama e bolokehile 'me tharollo e arotsoe ka ho lekana ka har'a hlobo.
Ho tiisa Gel
Lumella gel hore e tiee mocheso oa kamore, oo hangata o nkang metsotso e 20-30 ho itšetlehile ka mahloriso le botenya ba gel.
Rho ntsha Leka
Hang ha gel e tiile ka ho feletseng, tlosa lekama ka hloko ho hlahisa liliba tsa mohlala. Beha gel hammoho le hlobo ka kamoreng ea electrophoresis 'me ue koahele ka tekanyo e loketseng ea electrophoresis buffer, ho etsa bonnete ba hore gel e kene ka ho feletseng.
Ho itokisetsa Electrophoresis
Ka mor'a hore gel e se e loketse, laela lisampole tsa hau lilibeng, 'me u tsoele pele ka teko ea electrophoresis.
Haeba u na le mathata ka ho lokisa li-gel, ikutloe u lokolohile ho ikopanya le rona. Re na le litsebi tsa lab tse fumanehang ho buisana le uena.
Re thabetse ho arolelana litaba tse monate: tanka ea rona e tsebahalang ea DYCP-31DN e horizontal electrophoresis e ntse e phahamisoa., ikopanye le rona bakeng sa tlhaiso-leseling e batsi hona joale!
DYCP-31DN tanka e otlolohileng ea electrophoresis
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) e ikhethetse ho etsa lisebelisoa tsa electrophoresis ka lilemo tse fetang 50 ka sehlopha sa rona sa litsebi tsa setsebi le setsi sa R&D. Re na le mohala o tšepahalang le o felletseng oa tlhahiso ho tloha ho moralo ho ea ho tlhahlobo, le ntlo ea polokelo, hammoho le tšehetso ea papatso. Lihlahisoa tsa rona tse ka sehloohong ke Electrophoresis Cell (tanka / kamore), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System joalo-joalo Re boetse re fana ka lisebelisoa tsa lab joaloka PCR sesebelisoa, vortex mixer le centrifuge bakeng sa laboratori.
Haeba u na le moralo oa ho reka lihlahisoa tsa rona, ka kopo u se ke oa qeaqea ho ikopanya le rona. U ka re romella molaetsa ka lengolo-tsoibila[imeile e sirelelitsoeng]kapa[imeile e sirelelitsoeng], kapa ka kopo re letsetse ho +86 15810650221 kapa eketsa Whatsapp +86 15810650221, kapa Wechat: 15810650221.
Ka kopo Skena khoutu ea QR ho e kenya ho Whatsapp kapa WeChat.
Nako ea poso: Oct-15-2024