Protein Electrophoresis Common Issues (2)

Re arolelane litaba tse ling tse tloaelehileng mabapi le lihlopha tsa electrophoresis pele, le rona'Ke rata ho arolelana liketsahalo tse ling tse sa tloaelehang tsa polyacrylamide gel electrophoresis ka lehlakoreng le leng. Ronaakaretsa litaba tsena bakeng sa bareki ba rona' sesupo sa ho fumana mabaka le hofumana liphetho tse nepahetseng le ho ntlafatsa boleng ba karohano ea protheine.

Gel eseng polymerized Ho ka etsahala

Mabaka ke:

(a) bohloeki bo sa lekaneng ba monomer, bo hlokang recrystallization.

(b) Oksijene e qhibilihisitsoeng ka har'a tharollo ea gel e thibela polymerization ea gel. Ho lokela hore ho sebelisoe pompo e sebetsang hantle ea sparging.

(c) Ammonium persulfate ha e sebetse kapa ha e lekane ka bongata. Lokisetsa bocha kapa sebelisa sehlopha se seng sa ammonium persulfate se nang le khatello e phahameng ea maikutlo.

 Tgel e se e le polymerized ntle le ho eketsa lera la metsi.

Mabaka ke:

(a) Folisa tharollo ea gel pele u eketsa mokelikeli ho fokotsa sekhahla sa polymerization.

(b) Fokotsa palo ea TEMED kapa ammonium persulfate e sebelisitsoeng.

(c) Ho potlakisa tšebetso.

Gel e thellela ka ntle ho phaposi ea electrophoresis ka mor'a polymerization

Sena se atisa ho etsahala ka li-gel tse tlaase haholo. Tharollo e 'ngoe ke ho phuthela lesela la dialysis ho pota-pota tlase ea tube kapa ho sebelisa porous polypropylene base.

Mohlala ha o fumanoe ka mor'a electrophoresis

Mabaka ke:

(a) Chelete ea mohlala e sa lekaneng. Eketsa palo ea sampole.

(b) Lintho tsa tharollo ea litšila kapa nako ea ho tsepamisa maikutlo kapa ea ho silafatsa ha ea lekana. Kenya sebaka sa tharollo ea litšila 'me u eketse nako ea ho tsepamisa mohopolo le ho senya nako.

(c) Mohlala o ile oa phaphamala nakong ea ho kenya. Eketsa boima ba tharollo ea sampole 'me u tšoare ka hloko.

(d) Khatiso ea gel ea karohano e phahame haholo, 'me sampuli ha ea ka ea kena ka gel. Fetola motsoako oa gel hantle.

(e) Khatiso ea gel ea karohano e tlase haholo, 'me sampole e entsoe ka electrophorese ho tsoa ho gel ea karohano. Fetola khatello ea gel le maemo a nepahetseng a electrophoresis.

(f) Haeba sampuli e le RNA, e ka 'na ea e-na le liprotheine tse etsang motsoako o moholo o thibelang li-pores tsa gel. Tlosa protheine ka botlalo ho tsoa sampoleng ea RNA.

(g) Mohlala o na le li-enzyme tse etsang hydrolyze sampole, 'me sampole e senyeha nakong ea electrophoresis. Hloekisa mohlala ka ho feletseng.

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Beijing Liuyi Biotechnology Company Ltd e etsa mefuta e mengata ea lihlahisoa tsa electrophoresis tse ka thusang ho rarolla mathata ao re ka kopanang le 'ona litekong tsa rona tsa electrophoresis. Khampani e thehiloe ka 1970. E ne e le ea naha 'me e hlahisa mochine oa ho tjheseletsa oa motlakase le mochine oa phallo ea indasteri ka nako eo. Ho tloha ka 1979, Beijing Liuyi Biotechnology Company Ltd e qala ho hlahisa lihlahisoa tsa electrophoresis. Hona joale khampaniis e mong oa ba etellang pelemoetsi oa lisebelisoa tsa laboratori le lisebelisoa tsa saense tse thehiloeng Beijing, Chaena. Ea eona letshwao la kgweboLIUYI e tumme Chaena sebakeng sena.

Lihlahisoa tsa k'hamphani li kenyelletsa lisebelisoa tse ngata tsa laboratori, ho kenyeletsoa tanka ea "nucleic acid electrophoresis" e otlolohileng, tanka / yuniti ea protheine ea electrophoresis, lebokose le letšo.mofuta UV analyzer,Gel Document Tracking Imaging Analyzer, le motlakase oa electrophoresis. Lihlahisoa tsena li sebelisoa haholo litsing tsa lipatlisiso, liunivesithing le liindastering tse fapaneng, joalo ka meriana, biotechnology, le tlhahlobo ea tikoloho.Khamphani ke k'hamphani e netefalitsoeng ea ISO9001 & ISO13485 mme e na le litifikeiti tsa CE.

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Ho na lemefuta e fapaneng eae otlolohilenglitanka tsa electrophoresis bakeng saprotheine electrophoresisbakeng sa tlhahlobo le ho tsebahatsa disampole tsa protheine ka polyacrylamide gel electrophoresis,lehape bakeng sa ho metha boima ba limolek'hule tsa disampole, ho hloekisa lisampole le ho lokisa lisampole.Lihlahisoa tsena kaofela li amoheloa ka haremaraka oa naha le mose ho maoatle.

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Motlakase oa motlakase oa electrophoresis ke karolo ea bohlokoa eatsamaiso ea electrophoresis, ho fana ka mohloli o tsitsitseng le o nepahetseng oa motlakase oa motlakase ho khanna mokhoa oa ho arohana.Itka tloaelo e fana ka motlakase o sa khaotseng kapa hona joale ho tsamaiso ea electrophoresis, ho itšetlehile ka protocol e khethehileng ea liteko. E boetse e lumella mosebelisi ho fetola motlakase kapa tlhahiso ea hajoale, hammoho le liparamente tse ling joalo ka nako le mocheso, ho ntlafatsa maemo a karohano bakeng sa teko e itseng.

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Fkapa ho shebella gel, o ka khetha letoto la UV Transilluminator WD-9403 le entsoeng ke Beijing Liuyi Biotechnology.A UV transilluminator ke sesebelisoa sa laboratori se sebelisetsoang ho bona le ho sekaseka lisampole tsa DNA, RNA le protheine. E sebetsa ka ho khantša lisampole ka lebone la UV, le etsang hore lisampole li khanye 'me li bonahale. Ho na le mefuta e mengata ea UV transilluminatore fanoang ke rona. WD-9403A ke ka ho khetheha bakeng sa ho shebella protheine electrophoresis, 'me WD-9403F e sebelisetsoa ho shebella DNA le protheine electrophoresis.

Letoto lena la lihlahisoa li ka sebetsa ho tloha ho li-gel ho ea ho li-gel ho latela litlhoko tsa hau tsa liteko.Re tsebise litlhoko tsa hau, OEM, ODM le barekisi ba amoheloa.We tla leka ka hohle ho u fa lihlahisoa le litšebeletso tsa rona.

Haeba u na le moralo oa ho reka lihlahisoa tsa rona, ka kopo u se ke oa qeaqea ho ikopanya le rona. U ka re romella molaetsa ka lengolo-tsoibila[imeile e sirelelitsoeng]kapa[imeile e sirelelitsoeng], kapa ka kopo re letsetse ho +86 15810650221 kapa eketsa Whatsapp +86 15810650221, kapa Wechat: 15810650221.


Nako ea poso: May-23-2023