Litaba tsa li-protein electrophoresis band li bua ka mathata kapa mekhoa e sa nepahaleng e ka hlahang nakong ea ho arola liprotheine ho latela tefiso ea bona ea motlakase ho sebelisa electrophoresis. Litaba tsena li ka kenyelletsa ponahalo ea lihlopha tse sa lebelloang kapa tse sa tloaelehang, tharollo e fosahetseng, ho tlotsa, kapa ho sotha ha lihlopha tsa protheine, har'a tse ling. Re akaretsa lintlha tse 'maloa tse tloaelehileng molemong oa bareki ba rona ho fumana mabaka le ho fumana liphetho tse nepahetseng le ho ntlafatsa boleng ba karohano ea protheine.
Pososelasehlopha- paterone ea sehlopha e kobehela holimo mahlakoreng ka bobeli a gel
Lebaka
1. Setsi sa gel se chesang ho feta mahlakore ka bobeli
2. Maemo a matla a feteletseng
Tharollo
① Buffer ha e kopane hantle kapa buffer phaposing e kaholimo e tsepamisitsoe haholo. Etsa buffer hape, ho netefatsa ho kopanya hantle, haholo ha o hlapolla 5x kapa 10x stock
② Fokotsa maemo a matla ho tloha ho 200 V ho isa ho 150 V kapa tlatsa phaposi e tlase ho isa ho 1 cm ho tloha holim'a poleiti e khuts'oane.
Ho tsitsa hoa protheine
Lebaka
1. Sample e tletse
2. Sample pula Tharollo
Tharollo
① Fokotsa sampole, ka khetho ea ho tlosa protheine e ka sehloohong sampoleng, kapa fokotsa matla a motlakase ka 25% ho fokotsa sekhahla.
② Centrifuge sampole pele o eketsoa ka SDS sampole buffer, kapa fokotsa% T ea gel
③ Karolelano ea SDS ho protheine e lokela ho lekana ho koahela molek'hule ka 'ngoe ea protheine ka SDS, ka kakaretso 1.4: 1. E ka hloka SDS e eketsehileng bakeng sa disampole tse ling tsa protheine ea membrane
Bletshekaletsengjala
Lebaka
1. Phallo ea liliba pele ho ho bulela hona joale
2. Matla a ionic a sampuli a tlase ho feta a gel
Tharollo
① Fokotsa nako lipakeng tsa ts'ebeliso ea mohlala le ho bulela motlakase
② Sebelisa buffer e tšoanang sampole joalo ka gel kapa gel ea stacking
Lihlopha tsa protheine tse sothehileng kapa tse khopameng
Lebaka
1. Polymerization e mpe ho potoloha liliba
2. Letsoai ka mohlala
3. Sebopeho sa gel se sa lekaneng
Tharollo
① Degas stacking gel tharollo ka botlalo pele ho ho lahla; eketsa ammonium persulfate le TEMED concentrations ka 25%, bakeng sa stacking gel kapa tlaase% T, tlohela APS e tšoanang le habeli mahloriso a TEMED.
② Tlosa letsoai ka dialysis, desalting;
③ Fokotsa sekhahla sa polymerization. Koahela li-gel ka hloko haholo.
Beijing Liuyi Biotechnology Company Ltd e etsa mefuta e mengata ea lihlahisoa tsa electrophoresis tse ka thusang ho rarolla mathata ao re ka kopanang le 'ona litekong tsa rona tsa electrophoresis.
Beijing Liuyi Biotechnology Company Ltd e thehiloe ka 1970, eo pele e neng e tsejoa e le Beijing Liuyi Instrument Factory, ke moetsi oa lisebelisoa tsa laboratori le lisebelisoa tsa mahlale tse thehiloeng Beijing, China. Ka boiphihlelo ba lilemo tse fetang 50 ho rala le ho etsa lihlahisoa tsa electrophoresis le lipatlisiso tsa mahlale a bophelo, e fetoha mofani ea ka sehloohong oa lisebelisoa tsa laboratori le tharollo naheng ea China. Lihlahisoa tsa k'hamphani li kenyelletsa lisebelisoa tse ngata tsa laboratori, ho kenyeletsoa tanka ea electrophoresis ea nucleic acid e otlolohileng, protheine e otlolohileng. tanka / yuniti ea electrophoresis, mofuta oa lebokose le letšo la UV analyzer, Gel Document Tracking Imaging Analyzer, le motlakase oa electrophoresis. Lihlahisoa tsena li sebelisoa haholo litsing tsa lipatlisiso, liunivesithing, le liindastering tse fapaneng, tse kang meriana, biotechnology, le tlhokomelo ea tikoloho.Khampani ke k'hamphani e netefalitsoeng ea ISO9001 & ISO13485 'me e na le litifikeiti tsa CE.
Ho na lemefuta e fapaneng eae otlolohilenglitanka tsa electrophoresis bakeng saprotheine electrophoresisbakeng sa tlhahlobo le ho tsebahatsa disampole tsa protheine ka polyacrylamide gel electrophoresis,lehape bakeng sa ho metha boima ba limolek'hule tsa disampole, ho hloekisa lisampole le ho lokisa lisampole.Lihlahisoa tsena kaofela li amoheloa ka haremaraka oa naha le mose ho maoatle.
Motlakase oa motlakase oa electrophoresis ke karolo ea bohlokoa eatsamaiso ea electrophoresis, ho fana ka mohloli o tsitsitseng le o nepahetseng oa motlakase oa motlakase ho khanna mokhoa oa ho arohana.Itka tloaelo e fana ka motlakase o sa khaotseng kapa hona joale ho tsamaiso ea electrophoresis, ho itšetlehile ka protocol e khethehileng ea liteko. E boetse e lumella mosebelisi ho fetola motlakase kapa tlhahiso ea hajoale, hammoho le liparamente tse ling joalo ka nako le mocheso, ho ntlafatsa maemo a karohano bakeng sa teko e itseng.
Fkapa ho shebella gel, o ka khetha letoto la UV Transilluminator WD-9403 le entsoeng ke Beijing Liuyi Biotechnology.A UV transilluminator ke sesebelisoa sa laboratori se sebelisetsoang ho bona le ho sekaseka lisampole tsa DNA, RNA le protheine. E sebetsa ka ho khantša lisampole ka lebone la UV, le etsang hore lisampole li khanye 'me li bonahale. Ho na le mefuta e mengata ea UV transilluminatore fanoang ke rona. WD-9403A ke ka ho khetheha bakeng sa ho shebella protheine electrophoresis, 'me WD-9403F e sebelisetsoa ho shebella DNA le protheine electrophoresis.
Letoto lena la lihlahisoa li ka sebetsa ho tloha ho li-gel ho ea ho li-gel ho latela litlhoko tsa hau tsa liteko.Re tsebise litlhoko tsa hau, OEM, ODM le barekisi ba amoheloa.We tla leka ka hohle ho u fa lihlahisoa le litšebeletso tsa rona.
Haeba u na le moralo oa ho reka lihlahisoa tsa rona, ka kopo u se ke oa qeaqea ho ikopanya le rona. U ka re romella molaetsa ka lengolo-tsoibila[imeile e sirelelitsoeng]kapa[imeile e sirelelitsoeng], kapa ka kopo re letsetse ho +86 15810650221 kapa eketsa Whatsapp +86 15810650221, kapa Wechat: 15810650221.
Nako ea poso: Mar-29-2023