Kakaretso e KhutšoanyaneeaAgarose Gel Electrophoresis
Agarose gel electrophoresis ke mokhoa o sebelisoang haholo ho baeloji ea limolek'hule bakeng sa karohano ea li-nucleic acid, joalo ka DNA le RNA, ho latela boholo ba tsona. Mokhoa ona o sebelisa gel e entsoeng ka agarose, e leng polysaccharide ea tlhaho e nkiloeng litlamong tsa leoatle. Gel e sebetsa e le sieve ea molek'hule, e lumellang limolek'hule hore li falle ka eona ha ho sebelisoa motlakase. Ts'ebetso ena ea karohano e thusa bafuputsi ho sekaseka le ho khetholla lisampole tsa asiti ea nucleic ka ho sheba lihlopha tse ikhethileng tse entsoeng ho latela boholo ba tsona. Agarose gel electrophoresis ke sesebelisoa sa mantlha sa mesebetsi e kang tlhahlobo ea sekhechana sa DNA, tlhahlobo ea bohloeki, le lipatlisiso tsa baeloji ea limolek'hule.
Ka tloaelo, tanka ea agarose gel electrophoresis e bitsoa tanka ea horizontal electrophoresis hape. Lisebelisoa tse kholo tsa agarose gel electrophoresis li kenyelletsa:
DNA Fragment Analysis:
Karohano le tlhahlobo ea likhechana tsa DNA tse ipapisitseng le boholo, tse bohlokoa bakeng sa mesebetsi e kang genotyping le DNA ea menoana.
Tlhahlobo ea RNA:
Qeto ea limolek'hule tsa RNA, tse thusang boithutong ba mofuta oa polelo ea liphatsa tsa lefutso le botšepehi ba RNA.
Netefatso ea Sehlahisoa sa PCR:
Netefatso ea lihlahisoa tsa polymerase chain reaction (PCR) ho netefatsa ho holisoa ka katleho le ho fumana boholo bo nepahetseng ba likhechana.
Tlhahlobo ea Plasmid DNA:
Tlhahlobo ea plasmid DNA bakeng sa taolo ea boleng, netefatso ea liteko tsa cloning, le ho tsebahatsa DNA e kopaneng.
Tekolo ea Bohloeki:
Boikemisetso ba bohloeki ba lisampole tsa nucleic acid ka ho bona lintho tse ka bakang litšila kapa litšila.
Patlisiso ea Biology ea Limolek'hule:
Sesebelisoa sa mantlha lilaboratoring tsa baeloji ea limolek'hule bakeng sa liteko tse fapaneng, ho kenyeletsoa litokisetso tsa tatellano ea DNA le tšilo ea li-enzyme tse thibelang.
Beijing Liuyi Biotechnology Co., Ltd. (eo pele e neng e le Beijing Liuyi Instrument Factory) e thehiloe ka 1970. Ke khoebo ea theknoloji e sebetsanang le tlhahiso ea lisebelisoa tsa tlhahlobo ea biochemistry le molecular biology analytical. , 'me e na le mohala o ka tšeptjoang le o feletseng oa tlhahiso ho tloha ho moralo ho ea ho tlhahlobo, le ntlo ea polokelo, hammoho le tšehetso ea papatso. Lihlahisoa tse ka sehloohong ke Electrophoresis Cell (tanka / kamore), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System joalo-joalo.
Re tsebise haholoanyane ka mohlala oa eona o le mong oa agarose gel electrophoresis-DYCP-31DN.
Selelekela sa aelectrophoresis ea gel ea garosetanka ea DYCP-31DN
| Boemo (LxWxH) | 310×150×120mm |
| Boholo ba Gel (LxW) | 60×60mm, 60×120mm, 120×60mm, 120×120mm |
| Kamo | 2+3 liliba, 6+3, 8+18 le liliba tse 11+25. |
| Botenya ba Kamano | 1.0mm, 1.5mm le 2.0mm |
| Palo ea Mehlala | 2-100 |
| Bolumo ea Buffer | 650ml |
| Boima ba 'mele | 1.0kg |
DYCP-31DN ke tanka ea Liuyi ea agarose gel electrophoresis, e nang le sesebelisoa sa ho akhela se fumanehang bakeng sa ho etsa li-gel tse 1-4. E ka etsa li-gel tse peli tse nyane ka boholo ba 60x60mm le gel e le 'ngoe e pharaletseng 120x60mm kapa gel e telele ea 60x120mm ka nako e le 'ngoe. Gel e kholo ka ho fetisisa eo e ka e etsang ke boholo ba 120x120cm.
Thebotenya ba kama e fanoangba1.0mm, 1.5mm le 2.0mm. Ka moralo oa li-electrode tse ntšitsoeng, ho bonolo haholo ho hloekisa le ho li boloka. "Moqapi oa borokho" o reretsoeng ho boloka tharollo ea buffer nakong ea ts'ebetso ea electrophoresis. Tšebeliso e nepahetseng ea buffer e bohlokoa bakeng sa ho boloka mabala a motlakase a tsitsitseng le ho netefatsa karohano e nepahetseng ea limolek'hule.
Likamoso tse ling tsa bohlokoa:
1) Likoahelo le litopo tse kholo tsa tanka (litanka tsa buffer) li pepenene, li bōpehile, li ntle, li tšoarella, li tiisa hantle, ha li na tšilafalo ea lik'hemik'hale; ha e na lik'hemik'hale, e hana khatello.
2) Li-electrode li entsoe ka platinum e hloekileng (tekanyo ea bohloeki ea tšepe e ntle ≥99.95%) e nang le likarolo tsa ho hanyetsa kutu ea electroanalysis le ho mamella mocheso o phahameng, ts'ebetso ea conduction ea motlakase e ntle haholo.
3) Mehlala ea liliba hangata ho thata ho e bona, kahoo sehlopha se setšo se tereing ea gel se etsa hore ho be bonolo ho shebella le ho kenya lisampole.
4) Matla a hloleha ha o bula sekoaelo bakeng sa polokeho ea mosebelisi.
Ho na le mefuta e mengata ea phepelo ea motlakase e fanoang bakeng sa DYCP-31DN. Electrophoresis e chesang ka ho fetisisa phepelo ea motlakase is DYY-6C. Rea u amohela ho etela sebaka sa rona sa Marang-rang bakeng sa mefuta e meng ea phepelo ea motlakase.
Bakeng sa ho bona teenagel ea agarose e nang leDNA kapa RNA, Liuyi e fana ka li-transilluminator tsa UV bakeng sa bareki.WD-9403C, WD-9403B leWD-9403X li fumaneha kaofela hore bareki ba li khethe. 
Agarose gel electrophoresis e thusa mesebetsing e kang ho hlahloba boholo ba likhechana tsa DNA, ho lekola bohloeki ba sampole ea DNA, kapa ho arola likhechana tse fapaneng tsa DNA bakeng sa tlhahlobo e tsoelang pele.
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Nako ea poso: Jan-11-2024