Electrophoresis ke mokhoa o sebelisoang ho arola li-biomolecules ho latela boholo ba tsona le tefiso ho sebelisa lebala la motlakase. E sebelisoa haholo ho saense ea baeloji ka merero e fapaneng, ho tloha ho tlhahlobo ea DNA ho isa ho tlhoekiso ea protheine. Mona, re hlahloba molao-motheo oa electrophoresis le mekhoa ea eona e fapaneng.
Molao-motheo oa Electrophoresis
Electrophoresis e itšetlehile ka ho tsamaea ha likaroloana tse qosoang tšimong ea motlakase. Setupo sa mantlha se kenyelletsa ho beha sampole (e nang le li-biomolecules tse qosoang) holim'a gel kapa ka tharollo, le ho sebelisa motlakase. Li-biomolecules li falla ka har'a sehare ka litekanyetso tse fapaneng ho latela tefiso le boholo ba tsona, e leng se bakang karohano.
Mefuta e meng ea Electrophoresis
1. Gel Electrophoresis
Agarose Gel Electrophoresis: E arola likaroloana tsa DNA le RNA ho latela boholo.
Polyacrylamide Gel Electrophoresis (PAGE): E rarolla liprotheine ho latela boholo le tefiso.
2. Capillary Electrophoresis
E sebelisa li-capillaries tse tšesaane bakeng sa karohano, e lumellang tlhahlobo e potlakileng ea DNA, RNA, le liprotheine.
Likopo ho Biology Sciences
1. Tlhahlobo ea DNA
Genotyping: E khetholla mefuta e fapaneng ea liphatsa tsa lefutso (mohlala, SNPs) e amanang le mafu.
DNA Sequencing: E khetholla tatellano ea li-nucleotide ka har'a molek'hule ea DNA.
DNA Fragment Analysis: Boholo ba likhechana tsa DNA bakeng sa ts'ebeliso ea baeloji ea limolek'hule.
2. Tlhahlobo ea RNA
RNA Electrophoresis: E arola limolek'hule tsa RNA bakeng sa tlhahlobo ea polelo ea liphatsa tsa lefutso le botšepehi ba RNA.
3. Tlhahlobo ea Liprotheine
SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis): E arola liprotheine ho latela boholo.
2D Electrophoresis: E kopanya isoelectric focusing le SDS-PAGE ho arola liprotheine ho latela ntlha ea isoelectric le boholo.
4. Tlhoekiso
Preparative Electrophoresis: E hloekisa li-biomolecules (mohlala, liprotheine) ho latela tefiso le boholo.
5. Likopo tsa Clinical
Hemoglobin Electrophoresis: Ho hlahloba hemoglobinopathies (mohlala, lefu la sickle cell).
Serum Protein Electrophoresis: E supa ho se tloaelehe ha liprotheine tsa serum.
6. Likopo tsa Forensic
DNA Profileing: E bapisa lisampole tsa DNA bakeng sa lipatlisiso tsa forensic.
Melemo ea Electrophoresis
Qeto e Phahameng: E arola li-biomolecules ho latela boholo le tefiso ka nepo e phahameng.
Mefuta-futa: E sebetsa ho DNA, RNA, liprotheine, le li-biomolecule tse ling tse qosoang.
Quantitative Analysis: E lekanya bongata ba biomolecules ho ipapisitsoe le matla a sehlopha.
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Nako ea poso: May-28-2024