Ho lokisa Gel ea Agarose Bakeng sa Electrophoresis

Ho lokisetsa Gel ea Agarose bakeng sa Electrophoresis

Tlhokomeliso: Apara liatlana tse lahloang kamehla!

Litaelo ka Mohato

Ho lekanya phofo ea Agarose:use boima ba pampiri le tekanyo ea elektronike ho lekanya 0.3g ea phofo ea agarose (e thehiloeng tsamaisong ea 30ml).

Ho lokisa TBST Buffer:plokisa 30ml ea 1x buffer TBST ka botlolong ea 100ml Erlenmeyer.

Ho qhibiliha phofo ea Agarose:prona phofo ea agarose ka har'a buffer ea TBST 'me u sisinyehe hantle.Li kenye kahareho microwave le mocheso (hangata bakeng sa metsotsoana e 50, e koahetsoeng ka foil ea aluminium) ho fihlela e qhibiliha ka ho feletseng.

Ho Pholisa le ho Eketsa Nuclease:use gloves ho tlosa motsoako ho tswa ho microwave le ho o tlohela hanyenyane metsing a batang ho fihlela o futhumala (hoo e ka bang 60 ° C). Kenya 2µl ea nuclease (Eb substitute) ha u ntse u sisinyeha ho kopanya hantle.

Ho lokisetsa Gel Mold:

• Ctšetleha le ho omisa theterei ea gel le sesebelisoa sa ho lahla li-gelka tanka ea electrophoresis.
•  Place geltereika tanka e ka hare ebe o kenya kama ka sebakeng se tsitsitseng.
• Kopanya tharollo ea gel ea agarose, e pholileng hoo e ka bang 65 ° C, 'me ue tšollele ka hlokoterei ea gelhosesebelisoa sa ho lahla li-gel, ho e hasanya butle ho fihlela e etsa lera le lekanang la gel holim'a poleiti ea khalase.
• E lumelle hore e lule mocheso oa kamore ho fihlela gel e tiisa ka ho feletseng.
• Tlosa lekama ka bonolo 'me u tlose theipi.
• Beha geltereika tanka ea electrophoresis.

Bohlokoa: Netefatsa hore ha ho na lipululana tsa moea sebakeng sa meno a khekhe. Bokaholimo ba mokelikeli bo lokela ho ba boreleli ntle le maqhubu.

Ho matha Gel

Loading the Gel

Ka mor'a hore gel e tiee, e behe ka tanka ea electrophoresis 'me u tšollele "electrophoresis buffer" ho fihlela gel e qoelisoa.

Ho Lokisetsa Mehlala

• Nka lesupa 'me u laele buffer ho tloha sehatsetsing.
• Kenya 6µl ea buffer ho lisampole 'me u kopanye hantle.
• U sebelisa micropipette, butle-butle beha lisampole ka har'a liliba tse kholo tsa gel (e-ba hlokolosi hore u se ke ua phunya gel 'me u se ke ua ntša molumo oohle ho qoba lipululo tsa moea).
• Kenya letšoao ka har'a liliba life kapa life tse nyane (hopola boemo ba eona).

Ho qala ka Electrophoresis

• Koahela tanka ea electrophoresis 'me u qale electrophoresis hang ka mor'a ho kenya gel.
• Beha motlakase ho 60-100V. Mehlala e tla falla ho tloha ho electrode e mpe (e ntšo) ho ea ho electrode e ntle (e khubelu).
• Motlakase o phahameng o khutsufatsa mefuta e fapaneng ea karohano ea gel ea agarose.
• Emisa electrophoresis ha dae e putsoa ea bromophenol e fihla hoo e ka bang 1cm ho tloha moeling o ka tlase oa poleiti ea gel.

Ho Sheba Liphetho

Ka mor'a ho arola lihlopha, emisa electrophoresis, ntša gel, 'me u bone ka ho toba le ho e nka lifoto.

Sebelisa mokhoa oa ho nka litšoantšo ka li-gel ho nka lifoto le ho shebella lihlopha (hlahloba hore na ho na le lihlopha tsa lesupa kapa lisampole).

Kamora ho fumana 'mapa oa sehlopha sa gel, fumana lesupa. Ho ipapisitsoe le lesupa, o ka tseba hore na ke lihlopha life tse shebiloeng!

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) e entsoe lihlahisoa tsa electrophoresis ka lilemo tse fetang 50. Re hlahisa litsamaiso tsa rona tse rapameng tsa electrophoresis mona bakeng sagel ea agaroseelectrophoresis.Khethamekhoa e otlolohileng ea electrophoresisho tsoa Beijing liuyi bakeng sa teko ea hau ea gel electrophoresis ho thusa lipatlisiso tsa hau indastering ea biotechnology.

Re fana ka mefuta e fapaneng ea litanka tse otlolohileng tsa electrophoresis bakeng sa ho lahla le ho matha hanyaneagaroseli-gel ho isa ho tse kholoagaroseli-gel

Tataiso ea Khetho bakeng sa Yuniti ea Horizontal Immersed Gel Electrophoresis

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) e ikhethetse ho etsa lisebelisoa tsa electrophoresis ka lilemo tse fetang 50 ka sehlopha sa rona sa litsebi tsa setsebi le setsi sa R&D. Re na le mohala o tšepahalang le o felletseng oa tlhahiso ho tloha ho moralo ho ea ho tlhahlobo, le ntlo ea polokelo, hammoho le tšehetso ea papatso. Lihlahisoa tsa rona tse ka sehloohong ke Electrophoresis Cell (tanka / kamore), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System joalo-joalo Re boetse re fana ka lisebelisoa tsa lab joaloka PCR sesebelisoa, vortex mixer le centrifuge bakeng sa laboratori.

Haeba u na le moralo oa ho reka lihlahisoa tsa rona, ka kopo u se ke oa qeaqea ho ikopanya le rona. U ka re romella molaetsa ka lengolo-tsoibila[imeile e sirelelitsoeng]kapa[imeile e sirelelitsoeng], kapa ka kopo re letsetse ho +86 15810650221 kapa eketsa Whatsapp +86 15810650221, kapa Wechat: 15810650221.

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