Lefapheng le ntseng le tsoela pele le ntseng le tsoela pele la baeloji ea limolek'hule, Polymerase Chain Reaction (PCR) le Gel Electrophoresis li hlahile e le mekhoa ea motheo e thusang ho ithuta le ho sebetsana le DNA. Mekhoa ena ha e bohlokoa feela lipatlisisong empa hape e na le ts'ebeliso e atileng ho tsa tlhahlobo ea mafu, mahlale a forensic, le biotechnology.
Setšoantšo se tsoang sebakeng sa marang-rang biology4alevel
PCR ke mokhoa oa phetoho o ntlafalitsoeng ke Kary Mullis ka 1983, o lumellang bo-ramahlale ho holisa karolo e itseng ea DNA ka mokhoa o hlakileng. Ts'ebetso e qala ka denaturation ea DNA, moo DNA e nang le likarolo tse peli e futhumatsang hoo e ka bang 94 ° C, e leng se etsang hore e arohane ka likhoele tse peli tse le 'ngoe. Sena se lateloa ke annealing, moo li-primers-li-nucleotide tse khutšoanyane tsa tatellano ea li-nucleotide li kopanang le tatellano e tlatsanang ea DNA ea mohatla o le mong mocheso o tlaase (hangata o ka bang 55 ° C). Qetellong, mohato oa ho atolosa o etsahala ho 72 ° C, moo enzyme ea DNA polymerase e kopanyang lesela le lecha la DNA ka ho eketsa li-nucleotide ho li-primers. Potoloho ena e phetoa ka makhetlo a 20-40, e lebisang ho limilione tsa likopi tsa tatellano ea DNA e lebisitsoeng.
Hang ha DNA e se e matlafalitsoe, Gel Electrophoresis e sebelisoa ho arola le ho hlahloba lihlahisoa tsa PCR. Mokhoa ona o kenyelletsa ho falla ha likhechana tsa DNA ka matrix a gel ea agarose tlas'a tšusumetso ea sebaka sa motlakase. Limolek'hule tsa DNA li qosoa hampe ka lebaka la mokokotlo oa tsona oa phosphate, 'me li fallela ho electrode e ntle. Gel e sebetsa joalo ka sefe, e lumella likaroloana tse nyane tsa DNA ho tsamaea kapele ho feta tse kholo. Ka lebaka leo, likhechana tsa DNA li arohanngoa ho latela boholo ba tsona, ka lihlopha tse ikhethang tse bonahalang tlas'a leseli la ultraviolet ka mor'a ho silafatsoa ka dae e kang ethidium bromide.
Beijing LIUYILihlahisoa tsa Gel Electrophoresis
Motsoako oa PCR le Gel Electrophoresis o matla lits'ebetsong tse ngata. Litlhahlobong tsa bongaka, PCR e sebelisetsoa ho bona boteng ba likokoana-hloko, liphetoho tsa lefutso, kapa tatellano e itseng ea DNA e amanang le lefu. Gel Electrophoresis ebe e lumella pono le netefatso ea likhechana tsena tsa DNA tse holisitsoeng. Ho mahlale a forensic, mekhoa ena e bohlokoa bakeng sa khatiso ea menoana ea DNA, moo e thusang ho bapisa lisampole tsa DNA tse tsoang litsing tsa botlokotsebe le tsa babelaelloa.
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) e ikhethetse ho etsa lisebelisoa tsa electrophoresis ka lilemo tse fetang 50 ka sehlopha sa rona sa litsebi tsa setsebi le setsi sa R&D. Re na le mohala o tšepahalang le o felletseng oa tlhahiso ho tloha ho moralo ho ea ho tlhahlobo, le ntlo ea polokelo, hammoho le tšehetso ea papatso. Lihlahisoa tsa rona tse ka sehloohong ke Electrophoresis Cell (tanka / kamore), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System joalo-joalo Re boetse re fana ka lisebelisoa tsa lab joaloka PCR sesebelisoa, vortex mixer le centrifuge bakeng sa laboratori.
Beijing LIUYILihlahisoa tsa Gel Electrophoresis
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Nako ea poso: Aug-15-2024