Protheine Blotting
Protein blotting, eo hape e bitsoang western blotting, ho fetisetsoa ha liprotheine ho li-membrane tse tiileng-mohato, ke mokhoa o matla le o tummeng oa ho bona le ho khetholla liprotheine.
Ka kakaretso, ts'ebetso ea ho thibela protheine e kenyelletsa khetho ea mokhoa o nepahetseng, lisebelisoa, membrane, buffer le maemo a phetisetso. Hang ha liprotheine li se li sa tsamaee ka lera, li fumaneha bakeng sa ho bonoa, ho bonoa le ho hlahlojoa.
Liuyi Biotechnology e fana ka boholo bo fapaneng ba sisteme e otlolohileng ea electrophoresis e ka fihlelang litlhoko tsa hau tsa liteko. Lisele tse ling tsa Liuyi tse otlolohileng tsa electrophoresis (litanka / likamore) li tsamaisana le kopano ea rona ea li-electrode bakeng sa ho fetisetsa molek'hule oa protheine ho tloha ho gel ho ea ho membrane litekong tsa blotting tsa bophirima.Thevertical electrophoresis tsamaisomohlalaDYCZ-24DN, DYCZ-25DleDYCZ-25Eli tsamaisana le mohlala oa sistimi ea phetisetso ea tankaDYCZ-40D, DYCZ-40GleDYCZ-40F. Phapang e kholo mabapi le litanka tsena ke boholo ba tanka bakeng sa molumo oa buffer, le boholo ba li-gel. Thevertical electrophoresis tsamaisomohlalaDYCZ-25De na le moralo oa ho senya mocheso bakeng sa ho pholisa hamolemo nakong ea ho sebetsa li-gel.
Mokhoa oa ho koala
Ho na le mekhoa e fapaneng ea ho fetisa, empa mokhoa o tummeng ka ho fetisisa ke ho fetisa electrophoretic, hobane e potlakile ebile e sebetsa ho feta mekhoa e meng. Ho na le litsela tse tharo tse ka fetisang protheine ho tloha SDS-PAGE kapa gel ho membrane: ho fetisoa ha tanka, ho fetisoa ha semi-dry le ho omella.Beijing liuyiBiotechnologye na lelihlahisoa bakeng saphetisetso ea tanka, phetiso ea semi-dry, 'me u re tsebise lintlha tse ling mabapi lephetiso ea tankalephetiso e omileng.
Sistimi ea phetisetso ea litanka - li-gel le li-membrane li qoelisoa ka har'a buffer ea phetisetso ka har'a litanka; litsamaiso tsena li na le thuso bakeng sa mosebetsi o mongata oa protheine, bakeng sa phetisetso ea protheine e sebetsang hantle le e ngata, le phetiso ea liprotheine tsa boholo bohle. Sistimi ea phetisetso ea litanka e fana ka maemo a bonolo haholo ha u khetha litlhophiso tsa motlakase, linako tsa ho koala le likhetho tsa ho pholisa.
Semi-dry system - li-gel le li-membrane li kentsoe lipakeng tsa lipampiri tsa sefa tse tletseng buffer tse amanang ka kotloloho le li-electrode tsa poleiti; litsamaiso tsena ka tloaelo li bonolo ho li theha ho feta litsamaiso tsa litanka 'me li na le thuso ha ho hlokahala hore ho fetele lintho tse ngata 'me ho sa hlokahale nako e telele ea phetisetso, kapa ha ho sebelisoa litsamaiso tse sa khaotseng tsa buffer. Likhetho tse sebetsang tsa ho pholisa li na le moeli ka ho koala semi-dry blotting.
DYCP-40C
BeijingLiuyi Biotechnology e ka fana ka litsamaiso tsa phetisetso ea litanka le litsamaiso tse ommeng tse omileng tseo u li khethang. MohlalaDYCZ-40D, DYCZ-40F,leDYCZ-40Gke litsamaiso tsa rona tsa phetisetso ea litanka; mohlalaDYCP-40CleDYCP-40Eke li-semi-dry system.
Letšoao la Liuyi le na le nalane ea lilemo tse fetang 50 Chaena mme k'hamphani e ka fana ka lihlahisoa tse tsitsitseng le tsa boleng bo holimo lefats'eng lohle.Nts'etsopele ea lilemo, e tšoaneloa ke khetho ea hau!
Ho fumana lintlha tse ling ka rona, ka kopo ikopanye le rona ka lengolo-tsoibila[imeile e sirelelitsoeng], thekiso01@ly.com.cn
Nako ea poso: Apr-08-2022