Molao-motheo
Cellulose acetate film electrophoresis ke mokhoa oa electrophoresis o sebelisang filimi ea cellulose acetate e le tšehetso. Ketsahalo eo ho eona likaroloana tse qosoang li eang ho eleketrode e fapaneng tlasa ts'ebetso ea tšimo ea motlakase e bitsoa electrophoresis. Kaha protheine e 'ngoe le e' ngoe e na le ntlha e itseng ea isoelectric, haeba protheine e kenngoa ka tharollo e nang le pH e ka tlaase ho ntlha ea isoelectric, protheine e tla qosoa ka mokhoa o nepahetseng ebe e ea ho electrode e fosahetseng. Ho fapana le hoo, e fallela palo e ntle. Hobane lebelo la limolek'hule tsa protheine tse tsamaeang tšimong ea motlakase li amana le tefo ea tsona, sebōpeho le boholo ba limolek'hule, liprotheine tse fapaneng li ka aroloa ke electrophoresis. Serum e na le mefuta e fapaneng ea liprotheine, tseo kaofela li nang le lintlha tsa isoelectric ho pH7.5 kapa ka tlase. Ha serum e behiloe pH 8.6 buffer ho tsamaisa electrophoresis, liprotheine tsohle tsa serum li qosoa hampe 'me li tla leba lehlakoreng le letle tšimong ea motlakase. Kaha liprotheine tse sa tšoaneng tsa serum li na le litefello tse fapaneng ka pH e tšoanang, likaroloana tsa molek'hule li fapane ka boholo, kahoo lebelo la ho falla le fapane, 'me li aroloa ke electrophoresis. Lintlha tsa isoelectric le boima ba limolek'hule tsa liprotheine tsa serum li bontšoa tafoleng e ka tlase:
| Protheine | Lintlha tsa Isoelectric(PI) | Boima ba molek'hule |
| Albumin | 4.88 | 69 000 |
| α1-gloulin | 5.00 | 200 000 |
| α2-gloulin | 5.06 | 300 000 |
| β-gloulin | 5.12 | 9 000 ~ 150 000 |
| γ-gloulin | 6.85~7.50 | 156 000 ~ 300 000 |
Teko ke ea ho arola liprotheine tse fapaneng serum ea mali le lera la cellulose acetate (abbr. CAM) joalo ka mecha ea litaba ea tšehetso. CAM ke mofuta oa li-loos tse foamedefilimi e nang le junifomo e ntle, 'me botenya ke 0.1mm-1.5mm, e nang le mokhoa o itseng oa ho monya metsi.
Lisebelisoa, lisebelisoa le li-reagents bakeng sa CAM Electrophoresis
Mehlala:serum ea mali ea motho ea phetseng hantle
Seletsa:phepelo ea motlakase DYY-6C, tanka ea electrophoresis DYCP-38C, sampole e phahameng ea ho kenya WD-9404
Li-reagents
1) cellulose acetate lera 7X9cm
2) PH 8.6 barbitol buffer solution (ionic matla 0.05-0.09, ke nako ea 0.06tid.): nka 1,84g Diethyl barbituric acid, ebe u nka 1.03g Diethyl sodium pentobarbital, eketsa metsi a mangata a qhibilihisitsoeng ho futhumatsa ho qhibiliha, 'me u etse ho fihlela ho 1000ml;
3) Letheba: Ponceau S 0.2g, Trichloroacetic 3g, eketsa 100ml metsi a silafetseng;
4) TBS / T kapa PBS / T: 45ml 95% ethanol, 5ml glacial acetic acid, eketsa 50ml metsi a silafetseng;
5) Tharollo ea ho Hloekisa: 70ml anhydrous ethanol, 30ml glacial acetic acid.
Mokhoa oa ho lekad
1) Lokisetsa lera: Kenya lesela ka har'a tharollo ea barbitol buffer 30min-8h, 'me ue ntše, tlosa tharollo e eketsehileng ka pampiri e monyang.
2) Ho kenya lisampole: Khetholla lehlakore le makukuno le lehlakore le boreleli la lera, 'me u tšoaee mola sebakeng sa 1.5cm ho isa pheletsong e kaholimo ka lehlakoreng le mahoashe. Laola lisampole ka sesebelisoa se holimo sa ho kenya lisampole ka lehlakoreng le thata. Tlhokomeliso: Mehlala e lokela ho kenngoa ka lehlakoreng le thata la lera. Ka mor'a hore lisampole tsa serum li kene ka ho feletseng ka har'a lera, fetola lera, beha lehlakore le thata (ka lisampole) le shebile fatše ho tanka, 'me qetello e nang le lisampole e behoa ka electrode e fosahetseng.
3) Electrophoresis: Bulela phepelo ea motlakase, 0.4～0.6m A / cm membrane, nako ea ho sebetsa ke 30-45 min. Ka mor'a ho matha electrophoresis, tima matla.
4) Silafatsa 'me u hloekise: Ntša lesela ka tankeng, 'me u qoeliseit ka har'a tharollo ea dae bakeng sa 5 min, ebe u e hloekisa ka tharollo ea ho hloekisa khafetsa ka makhetlo a 3-4 ho fihlela 'mala oa bokamorao o hlakile. Liprotheine tsa serum li lokela ho bontšoa lihlopheng, 'me ka tloaelo ho na le libaka tse hlano, ho theha holimo holimo ho moeli o tšoailoeng, Albumin, α1-gloulin, α2-gloulin, β-gloulin, γ-gloulin.
5) Ho boloka: beha electrophoresis e omilengsehlophaka ho hloekisa tharollo bakeng sa metsotso e 10-15, ebe oe ntša ebe o e khomarela ka khalase e hloekileng, ka mor'a hore e ome, e tla fetoha filimi e hlakileng.sehlopha.
TekoSephetho
Phello ea karohano ea lisampole tsa serum e ntle, 'me ha ho na ketsahalo ea band tailing. Ho pheta-pheta ha liphetho ho fapana ka lebaka la mekhoa ea liteko le mekhoa ea motho ea lekang, 'me ho pheta-pheta ho phahame.
Qetello
Sistimi e potlakileng ea tlhahlobo ea electrophoresis (Tanka ea electrophoresisDYCP-38C,phepelo ea motlakaseDYY-6C le mohlala o phahameng oa ho kenya WD-9404) o hlahisoang ke ronakhampani Beijing Liuyi Biotechnology Co., Ltde fihlela litlhoko tsa liteko,leliphetho li ka ikatisa, li bonolo ebile li potlakile, li loketseho rutalipatlisiso.
Beijing LiuyiBiotechnology Co., Ltd e sebetsa ka ho khetheha ho hlahiseng lihlahisoa tsa elecrtophoresis bakeng sa indasteri ea mahlale a bophelo, eoe na le nalane ea lilemo tse fetang 50 Chaena mme k'hamphani e ka fana ka lihlahisoa tse tsitsitseng le tsa boleng bo holimo lefatšeng ka bophara. Ho theosa le lilemo tsa nts'etsopele, e loketse khetho ea hau!
Hona joale re batla balekane, bobeli ba OEM electrophoresis tanka le baabi ba amoheloa.
Ho fumana lintlha tse ling ka rona, ka kopo ikopanye le rona ka lengolo-tsoibila[imeile e sirelelitsoeng]kapa[imeile e sirelelitsoeng].
Nako ea poso: Nov-14-2022