DNA ke engtatellano?
Ke mokhoa oa ho tsebatatellano e nepahetseng kapa tatellano ea metheo (A,C, G le T) ka har'a molek'hule ea DNA.Ke hobane’ng ha re lokela ho tseba tatellano ea DNA liphatseng tse itseng tsa lefutso? Mona re tseba lits'ebetso tse ling. Ea pele, e ka re thusa ho lemoha liphetoho tse bang teng liphatseng tse itseng tsa lefutso. Joale haeba re tseba tatelano ea DNA, ho tla re thusa ho khetholla sebōpuoa se seng ho se seng.li-haplotypesle dho khetholla li-polymorphisms.
Ho bohlokoa ebile ho bohlokoa ho etsa lipatlisiso ka mekhoa ea tatellano ea DNA. Ho la Beijing Liuyi Biotechnology Co., Ltd, re fana ka lisebelisoa tsa DYCZ-20 letoto la li-electrophoresis ho thusa bafuputsi ho hlahloba tatellano ea DNA, tlhahlobo ea menoana ea DNA, pontšo ea phapang jj.
Haufinyane tjena, setsebi lab ea rona se bonts'a mokhoa oa ho etsa gel ka lisebelisoa tsa rona tsa DYCZ-20F tsa electrophoresis. Setsebi sa Theknishine Mr.Wu o ile a arolelana mekhoa ea ho kenya gel ka phaposing ea khalase.
Sesebelisoa sa DYCZ-20F electrophoresis se ka lahlela boholo ba gel ea 340x450mm. Taba ea pele, re hloka ho lokisa mosebetsi oohle oo re o hlokang ho lahla gel, joalo ka tharollo ea gel, gpoleiti ea khale,gel botlolo, khekhe ea meno a shaka, spacer(0.4mm), likoto.
Pele re lahlela gel, re hloka ho hloekisa le ho lokisa poleiti ea khalase pele. Mona, mohlahlobi o khothalelitse ho sebelisa metsi ho hloekisa bokaholimo ba lipoleiti tsa khalase. Ebe o apesa Bind -silane holim'a poleiti ea khalase ho thusa gel ho khomarela poleiti ea khalase, ebe o apesa Repel-silane holim'anotched poleiti ea khalase ho ebola gel habonolo.
Mohlahlobi o bontšitse mekhoa e 'meli ea ho enta tharollo ea gel:
Sekama kamore ea khalase ea gel hanyane, 'me u ente tharollo ea gel ka ho lekana pele le pele. (Li-bubble tsa moea li lokela ho qojoa ho hlahisa nakong ea ts'ebetso ena. )
Haeba motho ea etsang liteko a khetha ka tsela ena, o lokela ho itloaetsa khafetsa ho tseba ka tsela ena.
Bakeng sa ba qalang, re khothaletsa mokhoa ona oa ho lahlela gel. Opa mahlakoreng a mararo ka likotoana, 'me u sekamise kamore ea khalase ea gel ka lehlakoreng la likhato tse 45, ebe u kenya tharollo ea gel ka lehlakoreng la hao le letona ho fihlela tharollo ea gel e tlala ka kamoreng eohle ea gel.
Ke lakatsa eka pontšo ena ea gel e ka thusa bareki ba rona ho itokisetsa liteko tsa bona.
Beijing Liuyi DYCZ-20 letoto la DNA sequence analysis electrophoresis sesebelisoa se sebelisetsoa ho lemoha liphetoho tsa lefutso. Phetoho ea liphatsa tsa lefutso ke e 'ngoe ea lisosa tse ka sehloohong tsa mafu a mangata a lefutso. Ka electrophoresis e hlahlobang DNA sampoleng, liphetoho tsa tatellano ea liphatsa tsa lefutso li ka bonoa, ka hona ho thusa lingaka ho tseba hore na mokuli o na le lefutso le bakang lefu. Letoto lena le ka sebelisoa bakeng sa genotyping. Genotyping e bolela tlhahlobo ea loci e itseng ka har'a genome ea motho ho fumana genotype ea bona. Sena ke sa bohlokoa ho bafuputsi hobane liphatsa tsa lefutso tse fapaneng li ka amahanngoa le ho ba le ts'oaetso ea mafu a itseng kapa karabelo ea lithethefatsi. Ka ho sebelisa sesebelisoa sa electrophoresis sa tatellano ea DNA ea Liuyi, genotyping e ka etsoa kapele le ka nepo, e fana ka motheo oa bohlokoa bakeng sa meriana ea motho ka mong. Ho feta moo, letoto lena la lisebelisoa le ka boela la sebelisoa bakeng sa tatellano ea DNA. Tatelano ea DNA ke mokhoa oa bohlokoa oa hore bafuputsi ba utloisise sebopeho le tšebetso ea genome. Ka ho beha lisampole tsa DNA tlhahlobisong ea electrophoretic, tatellano ea metheo ea tatellano ea DNA e ka khethoa, ho thusa bafuputsi ho hlalosa tlhahisoleseling ho genome. Rea u amohela ho ikopanya le rona ho fumana tlhaiso-leseling e batsi ka lihlahisoa.
Hona joale re batla balekane, bobeli ba OEM electrophoresis tanka le baabi ba amoheloa.
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Nako ea poso: Oct-12-2023