Gene Electroporator GP-3000
Litlhaloso
| Mohlala | GP-3000 |
| Mofuta oa Pulse | Exponential Decay le Square Wave |
| Phallo e phahameng ea motlakase | 401-3000V |
| Phaello e tlase ea motlakase | 50-400V |
| High voltage capacitor | 10-60μF ka mehato ea 1μF (10μF, 25μF, 35μF, 50μF, 60μF e khothalelitsoe) |
| Low voltage capacitor | 25-1575μF ka mehato ea 1μF (mehato e 25μF e khothalelitsoe) |
| Parallel resistor | 100Ω-1650Ω ho 1Ω mehato (50Ω e khothalelitsoe) |
| Phepelo ea motlakase | 100-240VAC50/60HZ |
| Mokhoa oa tšebetso | Taolo ea li-microcomputer |
| Nako e tsitsitseng | ka nako ea RC kamehla, e ka fetoha |
| Boima bo kahare | 4.5kg |
| Litekanyo tsa sephutheloana | 58x36x25cm |
Tlhaloso
Cell electroporation ke mokhoa oa bohlokoa oa ho hlahisa li-macromolecules tse tsoang kantle ho naha tse kang DNA, RNA, siRNA, liprotheine le limolek'hule tse nyane ka hare ho lera la sele.
Tlas'a tšusumetso ea sebaka se matla sa motlakase ka motsotsoana, lera la sele ka tharollo le fumana mokhoa o itseng oa ho phunyeletsa. Lintho tse qosoang tse tsoang kantle ho naha li kena lera la sele ka mokhoa o ts'oanang le electrophoresis. Ka lebaka la khanyetso e phahameng ea phospholipid bilayer ea lera la sele, maqhubu a bipolar a hlahisoang ke sebaka sa motlakase se kantle a tsamaisoa ke lera la sele, 'me matla a ajoang ho cytoplasm a ka hlokomolohuoa, hoo e batlang e se na hona joale ho cytoplasm. kahoo hape ho khetholla chefo e nyenyane ka mokhoa o tloaelehileng oa mokhoa oa electrophoresis.
Kopo
E ka sebelisoa bakeng sa electroporation ho fetisetsa DNA liseleng tse nang le bokhoni, lisele tsa limela le liphoofolo, le lisele tsa tomoso. Joalo ka electroporation ea libaktheria, tomoso, le likokoana-hloko tse ling, phetisetso ea lisele tse anyesang, le phetisetso ea lisele tsa semela le li-protoplasts, tlhahiso ea lisele le fusion ea liphatsa tsa lefutso, kenyelletso ea liphatsa tsa lefutso bakeng sa ho ngola le ho supa sepheo, ho hlahisoa ha lithethefatsi, liprotheine, li-antibodies, le limolek’hule tse ling ho ithuta sebōpeho sa lisele le tšebetso ea tsona.
Sebopeho
• Bokhoni bo phahameng: nako e khutšoanyane ea ho fetola, sekhahla se phahameng sa phetoho, ho pheta-pheta ho phahameng;
• Polokelo e bohlale: e ka boloka litekanyetso tsa liteko, tse loketseng basebelisi ho li sebetsa;
• Taolo e nepahetseng: phepelo e laoloang ke microprocessor-control;Ø
• Ponahalo e ntle: moralo o kopantsoeng oa mochine oohle, pontšo e hlakileng, ts'ebetso e bonolo.
LBH
P: Gene Electroporator ke eng?
A: Gene Electroporator ke sesebelisoa se sebelisetsoang ho hlahisa liphatsa tsa lefutso tsa exogenous, tse kang DNA, RNA, le liprotheine, ka har'a lisele ka mokhoa oa electroporation.
P: Ke mefuta efe ea lisele tse ka lebisoang ho Gene Electroporator?
A: Gene Electroporator e ka sebelisoa ho hlahisa liphatsa tsa lefutso ka mefuta e fapaneng ea lisele tse kenyeletsang libaktheria, tomoso, lisele tsa limela, lisele tsa mammalian, le likokoana-hloko tse ling.
P: Lisebelisoa tsa mantlha tsa Gene Electroporator ke life?
A:
• Electroporation ea baktheria, yeast, le likokoana-hloko tse ling: Bakeng sa phetoho ea liphatsa tsa lefutso le lithuto tsa tšebetso ea liphatsa tsa lefutso.
• Phetisetso ea lisele tse anyesang, lisele tsa semela, le li-protoplasts: Bakeng sa tlhahlobo ea liphatsa tsa lefutso, liphatsa tsa lefutso tse sebetsang, le boenjiniere ba liphatsa tsa lefutso.
• Kenyelletso ea ho kopanya lisele le liphatsa tsa lefutso: Bakeng sa ho theha lisele tse nyalisitsoeng le ho hlahisa liphatsa tsa lefutso.
• Kenyelletso ea liphatsa tsa lefutso tsa matšoao: Bakeng sa ho tšoaea le ho sala morao liphatsa tsa lefutso liseleng.
• Kenyelletso ea litlhare, liprotheine, le masole a 'mele: Bakeng sa ho batlisisa sebopeho sa lisele le tšebetso, phano ea lithethefatsi, le lithuto tsa tšebelisano ea liprotheine.
P: Gene Electroporator e sebetsa joang?
A: Gene Electroporator e sebelisa molumo o mokhuts'oane, o nang le matla a phahameng a motlakase ho etsa li-pores tsa nakoana ka har'a lera la sele, e lumellang limolek'hule tse sa tloaelehang ho kena ka seleng. Lera la sele le khutlela morao ka mor'a ho otla ha motlakase, le tšoara limolek'hule tse hlahisitsoeng ka har'a sele.
P: Melemo ea ho sebelisa Gene Electroporator ke efe?
A: Ho pheta-pheta ho phahameng le ho sebetsa hantle, ts'ebetso e bonolo: Ts'ebetso e bonolo le e potlakileng, taolo ea bongata, ha ho na genotoxicity: Tšenyo e nyane e ka bang teng ho liphatsa tsa lefutso tsa sele.
P: Na Gene Electroporator e ka sebelisoa bakeng sa mefuta eohle ea liteko?
A: Le hoja Gene Electroporator e sebetsa ka litsela tse ngata, katleho ea eona e ka fapana ho itšetlehile ka mofuta oa sele le liphatsa tsa lefutso tse hlahisoang. Ho bohlokoa ho ntlafatsa maemo bakeng sa teko ka 'ngoe e khethehileng.
P: Ke tlhokomelo efe e khethehileng e hlokahalang ka mor'a kenyelletso?
K: Tlhokomelo ea ka mor'a kenyelletso e ka kenyelletsa ho kenya lisele sebakeng sa ho hlaphoheloa ho li thusa ho lokisa le ho tsosolosa mesebetsi e tloaelehileng. Lintlha li ka fapana ho latela mofuta oa sele le liteko.
P: Na ho na le lipelaelo tsa polokeho mabapi le ho sebelisa Gene Electroporator?
A: Mekhoa e tloaelehileng ea tšireletso ea laboratori e lokela ho lateloa. Gene Electroporator e sebelisa motlakase o phahameng, ka hona ho tlameha ho lateloe mekhoa e nepahetseng ea ho sebetsana le polokeho ho thibela likotsi tsa motlakase.
