Electrophoresis Cell bakeng sa SDS-PAGE le Western Blot

Tlhaloso e Khutšoanyane:

DYCZ-24DN ke ea protheine electrophoresis, athe DYCZ-40D ke ea ho fetisetsa molek'hule oa protheine ho tloha gel ho ea ho membrane joalo ka lera la nitrocellulose tekong ea WesternBlot. Mona re na le motsoako o phethahetseng bakeng sa bareki ba rona ba ka kopanang le ts'ebeliso eo liteko li ka sebelisang tanka e le 'ngoe ho e etsagel electrophoresis, ebe o fapanyetsana mochine oa electrode ho etsa teko ea ho thibela ka tanka e tšoanang ea DYCZ-24DN. Seo u se hlokang ke sistimi ea DYCZ-24DN feela hammoho le module ea DYCZ-40D Electrode e tla u lumella ho fetoha kapele le ha bonolo ho tloha ho mokhoa o mong oa electrophoresis ho ea ho o mong.

Re romelle lengolo-tsoibila

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Tlhaloso

Boemo (L×W×H)	140×100×150mm
Boholo ba Gel (L×W)	75 × 83 limilimithara
Kamo	liliba tse 10 le liliba tse 15
Botenya ba Kamano	1.0mm le 1.5mm (E Tloaelehileng) 0.75mm (Ha u rata)
Palo ea Mehlala	20-30
Bolumo ea Buffer	400ml
Boima ba 'mele	1kg

Tlhaloso

DYCZ-24DN ke sele e emeng ea electrophoresis (tanka / kamore) bakeng sa SDS-PAGE, Native PAGE joalo-joalo Protein electrophoresis. Sele ena e ka lahlela le ho tsamaisa gel sebakeng se le seng. E bonolo ebile e ikhethile, ho bonolo ebile ho bonolo ho kenya lisampole. Tanka e entsoe ka thepa ea boleng bo holimo ea polycarbonate, e tšoarellang haholo ebile e pepenene. Tanka ena e bonaletsang e etsa hore ho be bonolo ho bona gel ha u etsa liteko. DYCZ-24DN e na le li-electrode tse tlosoang tse bonolo bakeng sa tlhokomelo. Li-electrode li entsoe ka platinum e hloekileng (≥99.95%) e leng electrolysis-corrosion mme e mamella mocheso o phahameng.

Ka mor'a gel electrophoresis, ho ea ka tlhokahalo ea liteko, ka linako tse ling, mohlahlobi o hloka ho fetisetsa gel ho tšehetso e tiileng bakeng sa tlhahlobo e eketsehileng. E bitsoa blotting experiment, e leng mokhoa oa ho fetisetsa liprotheine, DNA kapa RNA ho sejari. E etsoa ka mor'a gel electrophoresis, ho fetisetsa limolek'hule ho tloha gel ho ea ho lera le thibang. Ka mor'a ho thijoa, liprotheine tse fetisitsoeng, DNA kapa RNA li bonoa ka mebala e mebala-bala (mohlala, ho silafala ha silevera ea liprotheine), pono ea autodiographic ea limolek'hule tse nang le radiolabelled (tse entsoeng pele ho blot), kapa ho ngoloa ka mokhoa o itseng oa liprotheine kapa nucleic acid. Ea morao-rao e etsoa ka li-antibodies kapa li-probes tsa hybridization tse tlamang feela limolek'hule tse ling tsa blot mme li na le enzyme e kopantsoeng le tsona. Ka mor'a ho hlatsoa hantle, ts'ebetso ena ea enzymatic ('me kahoo, limolek'hule tseo re li batlang ka har'a blot) li bonoa ka ho kenngoa ka mokhoa o nepahetseng, ho fana ka deposit e mebala-bala kapa chemiluminescent reaction e ngolisitsoeng ke filimi ea lifoto.

Bakeng sa phepelo ea motlakase bakeng sa sele ena e theohileng ea gel electrophoresis, re khothaletsa e 'ngoe ea li-electrophoresis tse laolang nako ea mofuta oa DYY-6C.

Kopo

Bakeng sa SDS-PAGE, Native PAGE electrophoresis le ho fetisetsa molek'hule ea protheine ho tloha gel ho ea ho lera.

Sebopeho

The DYCZ-24DN mini vertical gel electrophoresis cell for SDS-PAGE, Native PAGE electrophoresis e na le likarolo tse latelang:

•E entsoe ka polycarbonate ea boleng bo holimo, e ntle ebile e tšoarella, e bonolo ho e shebella;

• Ka ho lahlela gel sebakeng sa pele, ho khona ho lahlela le ho tsamaisa gel sebakeng se le seng, ho bonolo ebile ho loketse ho etsa li-gel, le ho boloka nako ea hau ea bohlokoa;

• Moralo o khethehileng oa foreimi ea wedge o ka lokisa kamore ea gel ka thata;

• Tanka e bōpiloeng e nang le li-electrode tse hloekileng tsa platinum;

• Ho bonolo ebile ho loketse ho eketsa disampole;

•E khona ho rgel e le 'ngoe kapa tse peli ka nako e le' ngoe;

• Boloka tharollo ea buffer;

• Moralo o khethehileng oa tanka ho qoba buffer le ho lutla ha gel;

•Li-electrode tse ntšitsoeng, tse bonolo ho li hlokomela le ho li hloekisa;

• Ho tima-auto ha sekwahelo se bulwa;

Mojule oa elektrode, o boetse o bitsoa 'mele oa Ts'ehetso bakeng sa phetisetso kapa kopano ea elektrode ke karolo ea mantlha ea sistimi ea blotting DYCZ-40D. E na le likarolo tsa 'mala o mofubelu le o motšo le li-electrode tse khubelu le tse ntšo ho etsa bonnete ba hore gel e tsamaisana hantle nakong ea phetisetso, le moralo o sebetsang hantle o nolofatsang ho kenya le ho tlosoa ha lik'hasete tsa gel ho tloha ho sehlopha se tšehetsang bakeng sa ho fetisoa (kopano ea electrode).